Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effect of ozone and Satureja khuzistanica extract on the shelf life and quality of ‎pomegranate arils cv. Malas Saveh‎

Document Type : Full Paper

Authors
Taghi Akbari 1
Mohammad Ali Askari Sarcheshmeh 2
Samad Nejad-Ebrahimi 3
1 Ph. D. Candidate, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran‎
2 Associate Professor, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran ‎
3 Associate Professor of Medicinal Plants Research Institute, Shahid Beheshti University, Tehran, Iran‎
10.22059/ijhs.2022.339576.2022
Abstract
Ready-to-use pomegranate arils can be a good alternative to fresh fruits, but they have a short shelf life, so a safe and healthy method is needed to maintain the quality of these arils during storage. For this purpose, the present study was conducted to investigate the effect of combined ozone treatment and Satureja khuzistanica extract on pomegranate arils of Malas Saveh cultivar during storage for 21 days in the cold storage at 4 °C. Factorial experiment with two factors of postharvest treatments (including seven treatments: 1) 1 ppm ozone; 2) 3 ppm ozone; 3) 1 ppm ozone + 0.5% S. khuzistanica extract; 4) 1 ppm ozone + 1% S. khuzistanica extract; 5) 3 ppm ozone + 0.5% S. khuzistanica extract; 6) 3 ppm ozone + 1% S. khuzistanica extract; and 7) control), and sampling time (zero (before storage), 7, 14 and 21 days after storing arils in cold storage) were performed with three repetitions. The results showed that the highest amount of titratable acid (TA) (1.15%), anthocyanin (126 ppm), total phenol (115.6 mg GAE g-1 FW), and vitamin C (30.5 mg 100 g FW) during the storage period was obtained from 1 ppm ozone treatment with 0.5% extract of S. khuzistanica. Also, pomegranate arils treated with 1 ppm ozone + 0.5% S. khuzistanica extract had the highest appearance quality, and the lowest population of mesophilic, and psychrophilic bacteria, yeasts and fungal infections. In general, the combined treatment of ozone 1 ppm + 0.5% S. khuzistanica extracts is recommended as a safe and healthy method for storing pomegranate arils in storage.
Keywords
Biochemical traits
microbial contamination
ozonated water
plant extract
storage
Subjects
  1. Abdolahi, A., Hassani, A., Ghosta, Y., Bernousi, I., & Meshkatalsadat, M. (2010). Study on the potential use of essential oils for decay control and quality preservation of ‘Tabarzeh’ table grape. Journal of Plant Protection Research, 50, 45-52.
  2. Aindongo, W.V., Caleb, O.J., Mahajan, P., Manley, M., & Opara, U. (2013). Effects of storage conditions on transpiration rate of pomegranate aril-sacs and arils. Talor and Francis Online Library, 31(1), 35-42.
  3. Alikhani, M., Sharifani, M., Azizi, M., Musavizadehr, S.J., & Rahim, M. (2009). Increasing shelf life and maintaining quality of strawberry (Fragaria ananassa ) with application of mucilage edible coating and plant essential oil. Journal of Agricultural Sciences and Natural Resources, 16 (2), 1-9.
  4. Almasaudi, N. M., Al-Qurashi, A. D., Elsayed, M. I., & Abo-Elyousr, K. A. (2022). Essential oils of oregano and cinnamon as an alternative method for control of gray mold disease of table grapes caused by Botrytis cinerea. Journal of Plant Pathology, 104(1), 317-328.
  5. Askari Sarcheshmeh, M. A., Babalar, M., Pirzad, F., Talaei, A. R., & Lesani, H. (2020). Impact of methyl jasmonate treatment on antioxidant system of pomegranate fruit cv "Malase" Saveh during storage. Iranian Journal of Horticultural Science, 51(3), 679-689 (in Persian).
  6. Buluc, O., & Koyuncu, M. A. (2020). Effects of intermittent ozone treatment on postharvest quality and storage Life of pomegranate. Ozone: Science and Engineering, 1-9.‏
  7. Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology, 94(3), 223-253.
  8. Chen, C., Zhang, H., Zhang, X., Dong, C., Xue, W., & Xu, W. (2020). The effect of different doses of ozone treatments on the postharvest quality and biodiversity of cantaloupes. Postharvest Biology and Technology, 163, 111124.‏
  9. De Santis, D., Garzoli, S., & Vettraino, A. M. (2021). Effect of gaseous ozone treatment on the aroma and clove rot by Fusarium proliferatum during garlic postharvest storage. Heliyon, 7(4), e06634.‏
  10. Farzaneh, M., Kiani, H., Sharifi, R., Reisi, M., & Hadian, J. (2015). Chemical composition and antifungal effects of three species of Satureja ( hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biology and Technology, 109, 145-151.‏
  11. Fazeli, M. R., Amin, G., Attari, M. M. A., Ashtiani, H., Jamalifar, H., & Samadi, N. (2007). Antimicrobial activities of Iranian Sumac and Avishane shirazi (Zataria multiflora) against some food -borne bacteria. Food Control, 18, 646-649.
  12. Ghasemnezhad, M., Zareh, S., Rassa, M., & Sajedi, R.H. (2013). Effect of chitosan coating on maintenance of aril quality, microbial population and PPO activity of pomegranate (Punica granatum cv. Tarom) at cold storage temperature. Journal of the Science of Food and Agriculture, 93(2), 368-374.
  13. Glowacz, M., Colgan, R., & Rees, D. (2015). Influence of continuous exposure to gaseous ozone on the quality of red bell peppers, cucumbers and zucchini. Postharvest Biology and Technology, 99, 1-8.
  14. Han, C., Zhao, Y., Leonard, S. W., & Traber, M. G. (2004). Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria× ananassa) and raspberries (Rubus ideaus). Postharvest biology and Technology, 33(1), 67-78.
  15. Hasheminya, S. M., Mokarram, R. R., Ghanbarzadeh, B., Hamishekar, H., Kafil, H. S., & Dehghannya, J. (2019). Development and characterization of biocomposite films made from kefiran, carboxymethyl cellulose and Satureja Khuzestanica essential oil. Food chemistry, 289, 443-452.‏
  16. Holcroft, D. M., Gil, M. I., & Kader, A. A. (1998). Effect of carbon dioxide on anthocyanins, phenylalanine ammonia lyase and glucosyltransferase in the arils of stored pomegranates. Journal of the American Society for Horticultural Science, 123(1), 136-140.
  17. Jamshidi, M., Shabani, E., Hashemi, Z., & Ebrahimzadeh, M. A. (2014). Evaluation of three methods for the extraction of antioxidants from leaf and aerial parts of Lythrum salicaria (Lythraceae). International Food Research Journal, 21(2), 783-788.
  18. Kalb, D., Lackner, G., & Hoffmeister, D. (2013). Fungal peptide synthetases: an update on functions and specificity signatures. Fungal Biology Reviews, 27(2), 43-50.‏

19.   Karaca, H., & Velioglu, Y.S. (2020). Effects of ozone and chlorine washes and subsequent cold storage on microbiological quality and shelf life of fresh parsley leaves.  Lebensmittel-Wissenschaft & Technologie, 127, 109421.‏

  1. Khan,, Sattar, A., Wahid, M., & Jan, M. (1985). Radiation preservation of dry fruits. Proc. Radiation Disinfestation of Food and Agricultural Products. Institutionin Tropical Agriculture. Hawaii, Honolulu, USA, 207.
  2. Khan, A.S., Singh, Z., Abbasi, A.M., & Swinny, E.E. (2008). Pre-or post-harvest applications of putrescine and low temperature storage affect fruit ripening and quality of ′Angelino′ plum. Journal of Science of Food and Agriculture, 88, 1686-1695.
  3. Kuşçu, A., & Pazır, F. (2004). Ozone application in food industry. The Journal of Food, 29(2), 123-29.
  4. Labbe, M., Alvaro, P. & Carmen, S. (2010). Antioxidant capacity and phenolic composition of juices from pomegranates stored in refrigeration. International Conference on Food Innovation- Food innova. Valencia .Spain, pp 430.
  5. Lopez, V., Conesa, A., & Artes, F. (2005). Shelf life and overall quality of minimally processed pomegranate arils modified atmosphere packaged and treated with UV-C. Postharvest Biology and Technology, 37, 174-185.
  6. Lv, Y., Tahir, I. I., & Olsson, M. E. (2019). Effect of ozone application on bioactive compounds of apple fruit during short-term cold storage. Scientia Horticulturae, 253, 49-60.
  7. Nasiri, M., Barzegar, M., Sahari, M.A., & Niakousari, M. (2018). Application of Tragacanth gum impregnated with Satureja khuzistanica essential oil as a natural coating for enhancement of postharvest quality and shelf life of button mushroom (Agaricus bisporus). International Journal of Biological Macromolecules, 106, 218-226.‏
  8. Özen, T., Koyuncu, M.A., & Erbaş, D. (2020). Effect of ozone treatments on the removal of pesticide residues and postharvest quality in green pepper. Journal of Food Science and Technology, 1-11.‏
  9. Paulikienė, S., Venslauskas, K., Raila, A., Žvirdauskienė, R., & Naujokienė, V. (2020). The influence of ozone technology on reduction of carrot loss and environmental impact. Journal of Cleaner Production, 244, 118734.‏
  10. Pirzad, F., Askari Sarcheshmeh, M. A., Babalar, M., Talaei, A., & Lesani, H. (2021). Effect of arginine and methyl jasmonate on chilling injury and nutritional quality of pomegranate fruit cv "Malase Saveh". Iranian Journal of Horticultural Science, 52(3), 541-552 (in Persian).
  11. Saidi, M. (2014). Antioxidant activities and chemical composition of essential oils from Satureja khuzestanica, Oliveria decumbens and Thymus daenensis. Journal of Essential Oil Bearing Plants, 17(3), 513-521.‏
  12. Sayyari, M., Babalar, M., Kalantari, S., Martínez-Romero, D., Guillén, F., Serrano, M., & Valero, D. (2011). Vapour treatments with methyl salicylate or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates. Food Chemistry, 124, 964-970.
  13. Selcuk, N., & Erkan, M. (2015). Changes in phenolic compounds and antioxidant activity of sour sweet pomegranates Cv. ‘Hicaznar’ during Long-term storage under modified atmosphere packaging. Postharvest Biology and Technology, 109, 30-39.
  14. Šernaitė, L., Rasiukevičiūtė, N., & Valiuškaitė, A. (2020). The extracts of cinnamon and clove as potential biofungicides against strawberry grey mould. Plants, 9(5), 613.‏
  15. Singleton, V.L., & Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158.
  16. Shehata, S. A., Abdeldaym, E. A., Ali, M. R., Mohamed, R. M., Bob, R. I., & Abdelgawad, K. F. (2020). Effect of some citrus essential oils on post-harvest shelf life and physicochemical quality of strawberries during cold storage. Agronomy, 10(10), 1466.
  17. Sogvar, O.B., Koushesh Saba, M., & Emamifar. (2016). Aloe vera and ascorbic acid coatings maintain postharvest quality and reduce microbial load of strawberry fruit. Postharvest Biology and Technology, 114, 29-35.
  18. Souza, L.P., Faroni, L.R.D., Heleno, F., Cecon, P.R., Gonçalves, T.D.C., Silva, G.J., & Prates, L. H.F. (2018). Effects of ozone treatment on postharvest carrot quality. LTW-Food Science and Technology, 90, 53-60.
  19. Stover, E.D., & Mercure, E.W. (2007). The pomegranate: a new look at the fruit of paradise. HortScience, 42(5), 1088-1092.
  20. Taheri, A., Behnamian, M., Dezhsetan, S., & Karimirad, R. (2020). Shelf life extension of bell pepper by application of chitosan nanoparticles containing Heracleum persicum fruit essential oil. Postharvest Biology and Technology, 170, 111313.‏
  21. Valero, D., Valverde, J.M., Martinez-Romero, D., Guillen, F., Castillo, S., & Serrano, M. (2008). The combination of modified atmosphere packaging with eugenol or thymol to maintain quality, safety and functional properties of table grapes. Postharvest Biology and Technology, 41, 317-327.
  22. Varasteh, F., Arzani, K., Barzegar, M., & Zamani, Z. (2012). Changes in anthocyanins in arils of chitosan-coated pomegranate (Punica granatum cv. Rabbab-e-Neyriz) fruit during cold storage. Food Chemistry, 130, 267–272.
  23. Wang, S.Y., & Gao, H. (2013). Effect of chitosan -based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria x aranassa). LWT-Food Science and Technology, 52, 71-79.
  24. Wei, Y., Wei, Y., Xu, F., & Shao, X. (2018). The combined effects of tea tree oil and hot air treatment on the quality and sensory characteristics and decay of strawberry. Postharvest Biology and Technology, 136, 139-144.
  25. Whangchai, K., Saengnil, K., & Uthaibutra, K. (2006). Effect of ozone in combination with some organic acids on the control of postharvest decay and pericarp browning of Longan fruit. Crop Protection, 25(8), 821-825.
  26. Xu, D., Shi, M., Jia, B., Yan, Z., Gao, L., Guan, W., & Zuo, J. (2019). Effect of ozone on the activity of antioxidant and chlorophyll degrading enzymes during postharvest storage of coriander (Coriandrum sativum). Journal of Food Processing and Preservation, 43(8), e14020.‏
  27. Zahran, A.A.H., Ramadan, A.H., & Ahmed, T.A.W. (2015). Effect of chitosan on biochemical composition and antioxidant activity of minimally processed-Wonderful pomegranate arils during cold storage. Journal of Applied Botany and Food Quality, 88, 241-248.
  28. Zhang, H., Li, K., Zhang, X., Dong, C., Ji, H., Ke, R., & Chen, C. (2020). Effects of ozone treatment on the antioxidant capacity of postharvest strawberry. RSC Advances, 10(63), 38142-38157.‏
Iranian Journal of Horticultural Science
Volume 53, Issue 4
Winter 2023
Pages 1045-1059

  • Receive Date 16 May 2022
  • Revise Date 13 August 2022
  • Accept Date 09 September 2022