Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

The effect of organic matter and type of culture medium on vegetative ‎characteristics, enzymatic activity and essential oil of lemon balm (Melissa ‎officinalis L.) under salinity stress

Document Type : Full Paper

Authors
Shima Rahmanian 1
Abdolhossein Aboutalebi Jahroomi 2
Mehdi Hosseinifarahi 3
1 Ph. D. Candidate of Horticultural Science, Yasooj Branch, Yasooj, Iran
2 Associate Professor of Horticulture, Jahroom Branch, Islamic Azad University, Jahroom, Iran
3 Associate Professor of Horticulture, Yasooj Branch, Islamic Azad University, Yasooj, Iran
10.22059/ijhs.2022.326008.1945
Abstract
In order to study the effect of organic matter and type of culture medium on vegetative characteristics, enzymatic activity and essential oil of lemon balm under salinity stress, a factorial experiment based on completely randomized design with three replications in Jahrom city of Fars province carried out. The first factor was the medium culture at three levels (compost + soil, vermicompost + soil and compost + vermicompost + soil (all in equal proportions)), the second factor was organic matter at two levels (humic acid (HA) and effective microorganisms (EM)( concentration of 5 per thousand) and the third factor was salinity at three levels (515, 3656 and 7312 μm / cm). The results showed that with increasing salinity, proline content and activity of catalase and peroxidase enzymes increased and plant height, leaf number, chlorophyll and percentage of essential oil decreased. Co-addition of vermicompost and compost to soil on growth and physiological traits was more effective than either of these two substances alone. In general, the use of organic matter and effective microorganisms in the culture medium containing soil+ compost+ vermicompost reduced the negative effects of salinity on lemon balm and the use of organic matter and effective microorganisms can improved plant tolerance under salinity stress conditions and increased the growth characteristics and essential oil content of this medicinal plant.
Keywords
Catalase
fresh and dry weight
humic acid
sodium chloride
vermicompost
  1. Abdipour, M., Hosseinifarahi, M., & Najafian, S. (2019). Effects of humic acid and cow manure biochar (CMB) in culture medium on growth and mineral concentrations of basil plant. International Journal of Horticultural Science and Technology, 6(1), 27-38.
  2. Adamipour, N., Heydarianpour, M. B., & Zarei, M. (2016). Evaluation of the application of vermicompost to reduce the destructive effects of salinity stress on the green carpet of the tall lawn Festuca arundinacea (Schreb.‘ Queen ’). Science and Technology of Greenhouse Crops, 7(25), 35-46. (In Farsi).
  3. Akhzari, D., & Pessarakli, M. (2016). Effect of drought stress on total protein, essential oil content, and physiological traits of Levisticum officinale Journal of Plant Nutrition, 39(10), 1365-1371.
  4. Alscher, R. G., Donahue, J. L., & Cramer, C. L. (1997). Reactive oxygen species and antioxidants: relationships in green cells. Physiologia Plantarum, 100(2), 224-233.
  5. Anwar, M., Patra, D. D., Chand, S., Alpesh, K., Naqvi, A. A., & Khanuja, S. P. S. (2005). Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation, and oil quality of French basil. Communications in Soil Science and Plant Analysis, 36(13-14), 1737-1746.
  6. Arancon, N. Q., Edwards, C. A., Bierman, P., Metzger, J. D., & Lucht, C. (2005). Effects of vermicomposts produced from cattle manure, food waste and paper waste on the growth and yield of peppers in the field. Pedobiologia, 49(4), 297–306.
  7. Asghari, A., Yousefirad, M., & Masumi, A. (2016). Effects of organic fertilizers of compost and vermicompost on qualitative and quantitative triats of Lemon verbena. Journal of Medicinal Plants, 15(58), 63-71.
  8. Atiyeh, R. M., Lee, S., Edwards, C. A., Arancon, N. Q., & Metzger, J. D. (2002). The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology, 84(1), 7-14.
  9. Banerjee, A., & Roychoudhury, A. (2017). Effect of salinity stress on growth and physiology of medicinal plants. In Medicinal Plants and Environmental Challenges (pp. 177-188). Springer.
  10. Barrett-Lennard, E. G. (2003). The interaction between waterlogging and salinity in higher plants: causes, consequences and implications. Plant and Soil, 253(1), 35-54.
  11. Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205-207.
  12. BeykKhormizi, A., Hosseini Sarghein, S., Sarafraz Ardakani, M. R., Moshtaghioun, S. M., & Mousavi Kouhi, S. M. (2019). Interaction of saline water and vermicompost on growth and absorption of some mineral nutrients in four landraces of fennel, Foeniculum vulgare. Journal of Water Research in Agriculture, 1(1), 39-529. (In Farsi).
  13. Bohlouli, M., Dehestani-Ardakani, M., Shirmardi, M., & Razmjoo, J. (2019). Effect of organic and biological fertilizers on some growth characteristics of evening primrose (Oenothera biennis) under salinity conditions. Environmental Stresses in Crop Sciences, 12(1), 263-280. (In Farsi).
  14. Bonacina, C., Trevizan, C. B., Stracieri, J., dos Santos, T. B., Goncalves, J. E., Gazim, Z. C., & de Souza, S. G. H. (2017). Changes in growth, oxidative metabolism and essential oil composition of lemon balm (Melissa officinalis) subjected to salt stress. Australian Journal of Crop Science, 11(12), 1665-1674.
  15. Bromand Sivieri, M., Heidary, M., Gholami, A., & Ghorbani, H. (2021). Effects of foliar application of nano iron oxide and biofertilizers on the activity of ‎antioxidant enzymes and some physiological characteristics of the root and aerial ‎parts in black cumin (Nigella sativa)‎. Iranian Journal of Horticultural Science, 51(4), 939-953. (In Farsi).
  16. Canellas, L. P., Olivares, F. L., Okorokova-Façanha, A. L., & Façanha, A. R. (2002). Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots. Plant Physiology (Bethesda), 130(4), 1951-1957.
  17. Choudhury, F. K., Rivero, R. M., Blumwald, E., & Mittler, R. (2017). Reactive oxygen species, abiotic stress and stress combination. The Plant Journal, 90(5), 856-867.
  18. Daly, M. J., & Stewart, D. P. C. (1999). Influence of “effective microorganisms”(EM) on vegetable production and carbon mineralization–a preliminary investigation. Journal of Sustainable Agriculture, 14(2-3), 15-25.
  19. Darzi, M. T. (2007). Effect of biofertilizers application on qualitative and quantitative yield of fennel (Foeniculum vulgare Mill.) in order to reach to a sustainable agroecosystem. Tarbiat Modares University, Tehran.
  20. Dashti, M., Kafi, M., Astaraei, A., & Zabihi, H. R. (2018). Investigation of yield and yield components response of Salvia leriifolia Benth to the bio-logical and organic Fertilizers. Zeitschrift Für Arznei-& Gewürzpflanzen, 23(2), 84-90.
  21. De Menezes, C. P., Queiroga, F., Guerra, S., Pinheiro, L. S., Trajano, V. N., & Pereira, F. D. O. (2015). Investigation of Melissa officinalis essential oil for antifungal activity against Cladosporium carrionii. International Journal of Tropical Disease & Health, 8(2), 49-56.
  22. Farahbakhsh, J., Najafian, S., Hosseinifarahi, M., & Gholipour, S. (2020). The effect of time and temperature on shelf life of essential oil composition of Teucrium polium L. Natural Product Research, 0(0), 1-5.
  23. Flexas, J., & Medrano, H. (2002). Drought‐inhibition of photosynthesis in C3 plants: stomatal and non‐stomatal limitations revisited. Annals of Botany, 89(2), 183-189.
  24. Garcia-Mina, J. M., Mora, V., Olaetxea, M., Baigorri, R., Fuentes, M., Garnica, M., San Francisco, S., Erro, J., Urrutia, O., & Casanova, E. (2012). Main mechanisms involved in the effects of humic substances on soil-plant systems. Agrociencia Uruguay, 16(3), 188-190.
  25. Gill, S. S., & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12), 909-930.
  26. Gohari, G., Mohammadi, A., & Duathi Kazemnia, H. (2019). Effect of vermicompost on some growth and biochemical characteristic of Dracocephalum moldavica under water salinity stress. Journal of Agricultural Science And Sustainable Production, 29(1), 151-168 (In Farsi).
  27. Gorgini Shabankareh, H., Sabouri, F., Saedi, F., & Fakheri, B. A. (2017). Effects of different levels of humic acid on growth indices and essential oil of lemon balm (Melissa officinalis) under different irrigation regimes. Crop Science Research in Arid Regions, 1(2), 166-176.
  28. Haghshenas, M., Nazarideljou, M. J., & Shokoohian, A. (2020). Phytochemical and quality attributes of strawberry fruit under osmotic stress of nutrient solution and foliar application of putrescine and salicylic acid. International Journal of Horticultural Science and Technology, 7(3), 263-278.
  29. Hakim, M. A., Juraimi, A. S., Hanafi, M. M., Ismail, M. R., Selamat, A., Rafii, M. Y., & Latif, M. A. (2014). Biochemical and anatomical changes and yield reduction in rice (Oryza sativa) under varied salinity regimes. BioMed Research International, 1-11.
  30. Hargreaves, J., Adl, M. S., Warman, P. R., & Rupasinghe, H. P. V. (2008). The effects of organic amendments on mineral element uptake and fruit quality of raspberries. Plant and Soil, 308(1), 213-226.
  31. He, J., Yang, B., Dong, M., & Wang, Y. (2018). Crossing the roof of the world: Trade in medicinal plants from Nepal to China. Journal of Ethnopharmacology, 224, 100-110.
  32. Hosseini Farahi, M., & Norouzinejad, M. (2016). Effect of vermicompost and Phosphate Barvar-2® biofertilizers on some quantitative charactrestics and elements absorption in green basil (Ocimum basilicm) in Gachsaran region. Journal of Plant Ecophysiology, 8(24), 160-172. (In Farsi).
  33. Hosseini, H., Mousavi Fard, S., Fatehi, F., & Qader, A. (2017). Phytochemical changes and morphophysiological traits of thyme (Thymus vulgaris CV Varico 3) under salinity stress. Journal of Medicinal Plants, 16(10), 22-33.
  34. Hosseinian, S. H., Ebrahimi-Pak, N. A., Yousefi, A., & Agdernejad, A. (2019). Effect of water stress and foliar application of humic acid on morphophysiological characteristics of safflower. Journal of Soil and Water Conservation Research, 26(1), 219-232. (In Farsi).
  35. Iriti, M., Scarafoni, A., Pierce, S., Castorina, G., & Vitalini, S. (2019). Soil application of effective microorganisms (EM) maintains leaf photosynthetic efficiency, increases seed yield and quality traits of bean (Phaseolus vulgaris) plants grown on different substrates. International Journal of Molecular Sciences, 20(9), 2-9.
  36. Javaid, A., & Bajwa, R. (2011). Effect of effective microorganism application on crop growth, yield, and nutrition in Vigna radiata (L.) Wilczek in different soil amendment systems. Communications in Soil Science and Plant Analysis, 42(17), 2112-2121.
  37. Khadem al-Husseini, Z., Jafarian, Z., Roshan, W., & Ranjbar, G. (2018). The effect of water salinity on the quantity and quality of biochemical compounds of Lemongrass (Melissa officinallis). Iranian Science Research, 12(3), 370-379. (In Farsi).
  38. Khalid, K. A., & Cai, W. (2011). The effects of mannitol and salinity stresses on growth and biochemical accumulations in lemon balm. Acta Ecologica Sinica, 31(2), 112-120.
  39. Khan, B. M., Hossain, M. K., & Mridha, M. A. U. (2014). Improving Acacia auriculiformis seedlings using microbial inoculant (Beneficial Microorganisms). Journal of Forestry Research, 25(2), 359-364.
  40. Kiani, Z., Esmaeilpour, B., Hadian, J., Soltani Toolarood, A. A., & Fathololumi, S. (2015). effect of organic fertilizers on growth properties nuterien absorption and essential oil yield ofmedicinal plant of Mentha spicata L). Journal of Plant Production Research, 21(4), 63-80 (In Farsi).
  41. Layeghhaghighi, M., & Abbaszadeh, B. (2022). Evaluation quantitative, qualitative traits and elements adsorption of lemon verbena (Lippia citriodora) under biochar, vermicompost and plant growth promoting rhizobacteria. Iranian Journal of Horticultural Science, 53(3), 667-679 (In Farsi).
  42. Liu, C., & Cooper, R. J. (2000). Humic substances influence creeping bentgrass growth. Carbon, 54(59), 41-51.
  43. Mahlouji, M., Mousavizadeh, S. F., & Karimi, H. (2001). Effect of drought stress and sowing date on pino bean grain yield and yield components. Journal of Agricultural and Natural Resources, 4(1), 57-67.
  44. Mason, M. G., Jha, D., Salt, D. E., Tester, M., Hill, K., Kieber, J. J., & Eric Schaller, G. (2010). Type‐B response regulators ARR1 and ARR12 regulate expression of AtHKT1; 1 and accumulation of sodium in Arabidopsis shoots. The Plant Journal, 64(5), 753-763.
  45. Mokhtarzadeh, S., Demirci, B., Goger, G., Khawar, K. M., & Kirimer, N. (2017). Characterization of volatile components in Melissa officinalis under in vitro conditions. Journal of Essential Oil Research, 29(4), 299-303.
  46. Moladoost, K., & Shahmoradi, M. (2020). Identification of challenges facing development of the medicinal plants sector in Iran. Iranian Journal of Medicinal and Aromatic Plants Research, 36(5), 748-762.
  47. Mona, Y., Kandil, A. M., & Swaefy Hend, M. F. (2008). Effect of three different compost levels on fennel and salvia growth character and their essential oils. Biological Sciences, 4, 34-39.
  48. Nadeem, S. M., Ahmad, M., Zahir, Z. A., Javaid, A., & Ashraf, M. (2014). The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology Advances, 32(2), 429-448.
  49. Narimani, R., Moghadam, M., Nemati, S., & Ghasemi Pir Balouti, A. (2019). Evaluation of salinity stress modulation using humic acid and ascorbic acid in the medicinal plant Dracocephalum moldavica Journal of Plant Research, 31(2), 971-955.
  50. Ndona, R. K., Friedel, J. K., Spornberger, A., Rinnofner, T., & Jezik, K. (2011). Effective micro-organisms’(EM): An effective plant strengthening agent for tomatoes in protected cultivation. Biological Agriculture & Horticulture, 27(2), 189-203.
  51. Pei-Sheng, Y., & Hui-Lian, X. (2002). Influence of EM Bokashi on nodulation, physiological characters and yield of peanut in nature farming fields. Journal of Sustainable Agriculture, 19(4), 105-112.
  52. Pouresmaeil, M., Ghorbani, M. ahlagha, & Khavarinzhad, R. (2005). Effect of salinity on germination, fresh and dry mass, ion content, proline, soluble sugar and starch content in Suaeda fruticosa. Wilderness, 10(2), 1-10.
  53. Pramanik, P., Ghosh, G. K., Ghosal, P. K., & Banik, P. (2007). Changes in organic–C, N, P and K and enzyme activities in vermicompost of biodegradable organic wastes under liming and microbial inoculants. Bioresource Technology, 98(13), 2485-2494.
  54. Rahdari, P., Tavakoli, S., & Hosseini, S. M. (2012). Studying of salinity stress effect on germination, proline, sugar, protein, lipid and chlorophyll content in purslane (Portulaca oleracea) leaves. Journal of Stress Physiology & Biochemistry, 8(1), 182-193.
  55. Ravi Kiran, T., & Aruna, H. K. (2010). Antioxidant enzyme activities and markers of oxidative stress in the life cycle of earthworm, Eudrilus eugeniae. Italian Journal of Zoology, 77(2), 144-148.
  56. Razzaghi, F., Ahmadi, S. H., Adolf, V. I., Jensen, C. R., Jacobsen, S., & Andersen, M. N. (2011). Water relations and transpiration of quinoa (Chenopodium quinoa) under salinity and soil drying. Journal of Agronomy and Crop Science, 197(5), 348-360.
  57. Rostami, G., & Moghadam, M. (2019). Effects of azomite on growth and some physiological and biochemical characteristics of basil under salt stress conditions. Journal of Plant Process and Function, 8(29), 299-311 (In Farsi).
  58. Sabouri, F., Sirusmehr, A. R., & Gorginieh Shabankareh, H. (2017). Effect of irrigation regimes and humic acid foliar application on some morphological and physiological characteristics of sunflower (Helianthus annuus). Iranian Plant Biology, 9(4), 13-24 (In Farsi).
  59. Said-Al Ahl, H. A. H., & Mahmoud, A. A. (2010). Effect of zinc and/or iron foliar application on growth and essential oil of sweet basil (Ocimum basilicum) under salt stress. Ozean Journal of Applied Sciences, 3(1), 97-111.
  60. Sanjari, M., Sirusmehr, A. R., & Fakheri, B. (2015). Effect of drought stress and humic acid on morphological traits, yield and anthocyanin content of sour tea (Hibiscus sabdarifa). Journal of Agricultural Ecology, 8(3), 346-358. (In Farsi).
  61. Setayeshmehr, Z., & Esmaeilzadeh, S. (2013). Effect of salt stress on some phonological and biochemical characteristics in Coriandrum sativum Journal of Plant Production, 20(3), 111–126.
  62. Sharif, M., Khattak, R. A., & Sarir, M. S. (2002). Effect of different levels of lignitic coal derived humic acid on growth of maize plants. Communications in Soil Science and Plant Analysis, 33(19–20), 3567–3580.
  63. Sharifiasl, R., Kafi, M., Saidi, M., & Kalateh J. (2020). The effect of humic acid on growth and some physiological responses in bermuda grass subjected to salinity stress. Iranian Journal of Horticultural Science, 51(2), 415-425 (In Farsi).
  64. Sirousmehr, A., Bardel, J., & Mohammadi, S. (2015). Changes of germination properties, photosynthetic pigments and antioxidant enzymes activity of safflower as affected by drought and salinity stresses. Journal of Crop Ecophysiology, 8(4), 517-533.
  65. Uma, B., & Malathi, M. (2009). Vermicompost as a soil supplement to improve growth and yield of Amaranthus species. Research Journal of Agriculture and Biological Sciences, 5(6), 1054-1060.
  66. Uyanik, M., & Gurbuz, B. (2014). Chemical diversity in essential oil compositions of leaf, herb and flower in lemon Balm (Melissa officinalis). Turkish Journal of Agricultural and Natural Science, 1(2), 210-214.
  67. Yadollahi, P., Bijani, M., Heidari, M., & Asgharipoor, M. . (2013). Effect of organic and biological fertilizers on quantitative and qualitative characteristics of cumin cyminum l. in Sistan region. Journal of Applied Research of Plant Ecophysiology, 1(4), 79-90.
Iranian Journal of Horticultural Science
Volume 53, Issue 3
Autumn 2022
Pages 691-706

  • Receive Date 18 July 2021
  • Revise Date 11 December 2021
  • Accept Date 05 February 2022