Identification of self-incompatibility alleles in some of Yazd province almond ‎genotypes by using PCR method‏ ‏

Document Type : Full Paper

Authors

1 M.Sc. Student, Faculty of Agriculture, Zabol University, Zabol, Iran

2 Associate Professor, Faculty of Agriculture and Natural Resources, Yazd University, Yazd, Iran

3 Associate Professor, Faculty of Agriculture, Zabol University, Zabol, Iran

4 Associate Professor, Temperate Fruits Research Center, Horticultural Science Research Institute(HSRI), Agricultural Research, ‎Education and Extension Organization (AREEO), Karaj, Iran

Abstract

One of the problems in almond production is self-incompatibility in this plant, which is considered as an important point of breeding. Self-incompatibility causes non-uniformity of harvesting time as well as some of garden management and pollination problems. Most cultivars of almonds have gametophytic self-incompatibility that is controlled by a multi-allelic gene locus. The fertilization inhibitor factor in this phenomenon, pollen tube growth stops in the middle of the style. The purpose of this research was identification and determination of the self-compatible genotype in the Yazd province.in this investigation better genotypes of almond were collected and after DNA extraction was done, in order to detect S alleles in different almond and some hybrid genotypes, the specific primer pairs, including AS1II-AmyC5R, ConF-ConR and Cebador2-Cebador8, were used in the polymerase chain reaction. In polymerase chain reaction, using the AS1II-AmyC5R and Cebador2-Cebador8 primers, the Sf allele with the size of 1200 base pairs was detected. Using the ConF-ConR pair of primer, the S1, S2, S3, S10, S11, S23, and S31 alleles were detected in the self-incompatible samples. Using AS1II-AmyC5R pair of primer, the known alleles of S3, Sf, S2, S1, S5, S10 S11 S23, and S13 were detected. The other bands obtained from the PCR were related to the known self-in-compatibility alleles that might be considered as new alleles.  According to the obtained results, S1, S2, S3, and S11alleles had the highest frequency and S5, S23, S10, S13and S31alleles respectively had the lowest frequency.

Keywords

References

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Iranian Journal of Horticultural Science
Volume 51, Issue 4
February 2021
Pages 797-808

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History

  • Receive Date: 25 June 2019
  • Revise Date: 17 August 2019
  • Accept Date: 27 August 2019

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