Effect of culture medium and growth regulator in meristem culture of sweet Cherry

Document Type : Full Paper

Authors

1 Ph.D. Candidate, Department of Horticultural Science, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

2 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

3 Associate Professor, Crop and Horticultural Science Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran

4 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran

Abstract

Regeneration through meristem culture is an efficient way to obtain virus-free plants. This study was conducted to effect of the medium and growth regulator on meristem cultivation in sweet cherry cultivars with factorial experiment based on completely randomized design with seven cultivars (Zard, Siah-E-Mashhad, Dovomres, Bing, Pishres, Takdaneh and Hajyousefi) and type of treatment in four levels of medium and growth regulator (M1H1: half MS without growth regulator, M2H1: complete MS without growth regulator, M1H2: half MS with growth regulator, M2H2: complete MS with growth regulator). Meristem explants (less than 1 mm) were isolated from shoot tips and were cultured in MS medium under controlled conditions; after third subculture (sixth week), meristem survival and activation After sixth subculture (third month), the multiplication rate (production shoot to active sample) and length of the shoot were measured. The results showed that there was no significant difference in survival index as well as multiplication rate and shoot length between cultivars, but this difference was significant between treatments. The highest survival percentage was obtained for Hajyosefi (52.4%), multiplication rate for Dovomres (2.2) and shoot length for Bing (7.9 cm) cultivar in MS medium with 1mg.l-1 BA growth regulator.

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References

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Iranian Journal of Horticultural Science
Volume 50, Issue 3
December 2019
Pages 587-594

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History

  • Receive Date: 22 January 2018
  • Revise Date: 27 June 2018
  • Accept Date: 30 June 2018

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