Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Study on enzymatic and biochemical changes in cucumber plant treated with potassium phosphite under Fusarium oxysporum f. sp. radicis-cucumerinum stress

Document Type : Full Paper

Authors
Sahar Heidarzade 1
Shahrokh Gharanjik 2
Ali Dehestani 3
Darioush Shahriari 4
1 Former M.Sc. Student, Faculty of Agriculture, Shahrood University of Technolgy, Iran
2 Assistant Professor, Faculty of Agriculture, Shahrood University of Technolgy, Iran
3 Assistant Professor, Genetics & Agricultural Biotechnology Institute of Tabarestan (GABIT), Iran
4 Instructor, Agriculture and Natural Resources Research Center of Tehran Province, Iran
10.22059/ijhs.2017.138752.897
Abstract
In this study, we evaluated the effects of five levels of potassium phosphite (KH2PO3) on Induced Systemic Resistance (ISR), and also defense related enzymes and metabolites activities during different days in cucumber plants inoculated with Fusarium oxysporum. The experiment was designed as a split-plot in time based on a completely randomized design with three replications. The activity of defense-related enzymes including, catalase (CAT), guaiacol peroxidase (GPX), superoxide dismutase (SOD) and also the accumulation of secondary metabolites such as lipid peroxidation (MDA) and hydrogen peroxide (H2O2), were evaluated in a period of 11 days after pathogen inoculation. Results revealed that defense enzymes activities and metabolites accumulations were significantly increased in plants treated with potassium phosphite in comparison to control. The highest concentration of CAT enzyme (18.7 U mg-1 protein) was measured in plants treated with 4 gr l-1 potassium phosphite (KPhi4) and 5 days after inoculation, that showed 2.81 fold increase in comparison with control (no inoculate with the pathogen). Also the highest concentration of enzymes GPX (233 U mg-1 protein) and SOD (10.8 U mg-1 protein) were recorded at 3 days after inoculation in plants treated with KPhi4 in comparison with control plants. Results showed that accumulation of H2O2 in plant tissues and it's time was in accordance with increasing of defense enzymes CAT, GPX and SOD and it would indicate that the H2O2 production is the prerequisite for production of defense-related enzymes. It was also revealed that MDA accumulation in KPhi4 treatment was less than other treatments, indicating reduced tissue damage and alleviated disease symptoms.
Keywords
Cucumber
defense enzymes
Fusarium oxysporum
potassium phosphite
  1. Adams, F. & Conral, J. P. (1953). Transition of phosphate to phosphate. Soil Science, 75, 361-371.
  2. Aebi, H. E. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126.
  3. Andreu, A., Guevara, M., Wolski, E., Daleo, G. & Caldiz, D.  (2006). Enhancement of natural disease resistance in potatoes by chemicals. Pest Management Science, 62, 162-70.
  4. Ariaei, A., Eskandari, M., Fazli, H. & Pirnia, M. (2014). The effect of different compounds in reducing the growth of phytophthora. AgriculturalResearch, 7, 20-22. (in Farsi)
  5. Blokhina, O. & Fagerstedt K. V. (2010). Oxidative metabolism, ROS and NO under oxygen deprivation. Journal of Plant Physioligy and Biochemistry, 48(5), 359-373.
  6. Boerjan, W., Ralph, J. & Baucher, M. (2003). Lignin biosynthesis. Annual Review of Plant Biology, 54, 519-46.
  7. Chance, B. & Maehly, A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 11, 764-755.
  8. Cherif, M., Benhamou, N. & Menzies, J. G. (1992).Silicon induced resistance in cucumber plant against Pythium ultimum. Physiological and Molecular Plant Pathology, 41, 411-425.
  9. Daayf, F., Ongena, M., Boulanger, R. N., Hadrami, E. I. & Belanger, R. R. (2000). Induction of phenolic compounds in two cultivars of cucumber by treatment of healthy and powdery mildew infected plants with extracts of Reynoutria sachalinensis. Jornal of Chemical Ecology, 26, 1579-1593.
  10. Davey, M. W., Stals, E., Panis, B., Keulemans, J. & Swennen, R. I. (2005). High throughput of malondialdehyde in plant. Analytical Biochemistry, 347(2), 201-207.
  11. Deliopoulos, T., Kettlewell, P. S. & Hare, M. C. (2010). Fungal disease suppression by inorganic salts: a review. Crop Protection, 29, 1059-1075.
  12. Desikin, R., Mackerness, S., Hancock, J. & Neill, S. J. (2001). Regulation of the Arabidopsis
    transcriptosome by oxidative stress. Journal of Plant Physiology, 127, 159-172.
  13. Dixon, D. P., Cummins, L., Cole, D. J. & Edwards, R. (1998). Glutathione-mediated detoxification systems in plants. Current Opinion in Plant Biology, 1, 258-266.
  14. Foyer, C. H. & Noctor, G. (2000). Oxygen processing in photosynthesis regulation and signaling. New Phytologist, 146 (3), 395-388.
  15. Giannopolitis, S. (1977). Superoxide dismutase. I. Occurrence in higher plants. Plant Physiology, 59, 309-314.
  16. Jackson, T., Burgess, T., Colquhoun, I. & Hardy, G. (2000). Action of the fungicide phosphate on eucalyptus marginata inoculated with phytophthora cinnamomi. Plant Pathology, 15, 235-238.
  17. Janda, T., Szalai, G., Tari, I. & Paldi, E. (1999). Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays l.) plants. Planta, 208, 175-180.
  18. Landschoot, P. & Cook, J. (2005). Sorting out the phosphonate products. Plant physiology, 12, 73-74.
  19. Lobato, M. C., Olivieri, F. P., Wolski, E. A., Daleo, G. R., Caldiz, D. O. & Andreu, A. B. (2008). Phosphite compounds reduce disease severity in potato seed tubers and foliage. European Journal of Plant Pathology, 122, 349-358.
  20. Lobato, M. C., Machinandiarena, M. F., Tambascio, C., Dosio, G. A. A., Caldiz, D. O., Daleo, G. R., Andreu, A. B. & Olivieri, F. P. (2011). Effect of foliar applications of phosphite on post-harvest potato tubers. Eur. J. Plant Pathol, 130, 155-163.  
  21. Machinan, D., Lobato, M. C., Feldman, M. L., Rauldaleo, G. & Andrex, A. B. (2012). Potassium phosphate primes defense responses in potato against phytophthora infestans. Plant physiology, 70, 120­-123.
  22. Molavi, E., Aminian, H., Etebarian, H. R. & Shahriari, D. (2009). Investigation the resistance of greenhouse-cucumber cultivars to Fusarium stem and root rot disease (Fusarium oxysporum f. sp. radicis-ucumerinum). Journal of Agriculture, 11, 177-189.
  23. Moor, U., Polldma, P., Tonutare, T., Karp, K., Starast, M. & Vool, E. (2009). Effect of phosphate fertilization on growth, yield and fruit composition of strawberries. Journal of Scientia Horticulturae, 119, 264-269.
  24. Morkunas, I. & Gemerek, J. (2007). The possible involvement of peroxidase in defense of yellow lupin embryo axes against Fusarium oxysporum. Plant Physiology, 164, 497-506.
  25. Neto, A. D., Prisco, J. T., Eneas-Filho, J., Abreu, C. E. B. & Gomes-Filho, E. (2005). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and saltsensitive maize genotypes. Environ Experimental Botany, 56, 87-94.
  26. Rakha, M. & Lu, S. (1990). Evaluation of phosphate rates against phytophthora root rot disease on cucumber. Plant Physiology, 20, 57-59.      
  27. Rickard, D. A. (2000). Review of phosphorus acid and its salts as fertilizer materials. Journal of Plant Nutrition, 23, 161-180.
  28. Sairam, R. K., Rao, K. V. & Srivastava, G. C. (2003). Differential response wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163, 1037-1046.
  29. Seckin, B., Turkan, I., Sekmen, A. H. & Ozfidan, C. (2010). The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barley grass) and Hordeum vulgare L. (Cultivated barley). Jornal of Environmental and Experimental Botany, 69, 76-85.
  30. Selote, D. S. & Khanna-Chopra, R. (2004). Drought-induced spikelet sterility is associated with an inefficient antioxidant defense in rice panicles. Plant Physiology, 121, 462-471.
  31. Sergiev, V., Alexieva, E. & Karanov, E. (1997). Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comptes Rendus de l Acade mie Blugare Scienes, 51, 121-124.
  32. Smilie, R., Dunstan, R., Grant, B., Giffith, J., Iser, J. & Niere, J. (1990). The mode of action of the antifungal agent phosphate. Plant physiology, 20, 185-192.
  33. Stewart, R. R. C. & Bewley, J. D. (1980). Lipid peroxidation associated aging of soybean axes. Plant Physiology, 65, 245-248.
  34. Thao, H. T. B. & Yamakawa, T. (2008). Phosphite: fungicide fertilizer or biostimulator. Journal of soil science and Plant Nutririon, 55, 228-234.
  35. Turkan, I. & Demiral, T. (2009). Recent developments in understanding salinity tolerance. Environmental and Exprimantal Botany, 67, 2-9.
  36. Vakalounakis, D. J. (1996). Root and stem rot of cucumber caused by Fusarium oxysporum f. sp. Radicis-cucumerinum f. sp. Nov. Plant D isease, 80, 313-316.
Iranian Journal of Horticultural Science
Volume 48, Issue 3 - Serial Number 3
Winter 2018
Pages 601-611

  • Receive Date 26 December 2015
  • Revise Date 30 May 2016
  • Accept Date 26 June 2016