Evaluate the effect of chitosan on the activities of antioxidant enzymes in Ajwain (Carum copticum L.) under drought stress

Document Type : Full Paper

Authors

1 Former M. Sc. Student, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran

2 Assistance Professor, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran

3 Asocciate Professor, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran

4 Instructor, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran

Abstract

Today, the use of chitosan as one of plant hormones to increase plant resistance to stresses such as drought has been increased. In order to evaluate the effect of different levels of chitosan on antioxidant enzyme activities in Ajwain under drought stress, the experiment was conducted as split plot based on randomized complete block design with Three replications in research farm of Zabol University in 2014. Drought as the main factor in three levels A1) 50 mm evaporation (control), A2) 100 mm evaporation (medium stress) and A3) 150 mm evaporation (severe stress) of pan evaporation class A and different levels of chitosan spray (control, 0.1, 0.5, 1, 2 g/L) were considered as Sub factor. In this study, by increasing period between two irrigations, the activities of Catalase, Peroxidase, Ascorbate Peroxidase, Gayacolperoxidase, and polyphenoixidase enzymes were increased. There was no significant difference between control and moderate stress for most parameters but maximum activities of Antioxidant Enzymes were observed in severe stress. Using different levels of chitosan caused to improve the activity of antioxidant enzymes, so that, the interaction effect of 0.5 g/l chitosan and drought stress had increased most parameters significantly than that for  control.

Keywords


  1. Agarwal, S. & Pandey, V. (2004). Antioxidant enzyme resposes to NaCl stress in Cassia angustifolia. - Biologia Plantarum, 48, 555-560.
  2. Agrawal, G., Rakwal, R., Tamogami, S., Yonekurad, M., Kubo, A. & Saji, H. (2002). Chitosan activates defense/stress response(s) in the leaves of Oryza sativa seedlings. Plant Physiology and Biochemistry, 40, 1061-1069
  3. Alscher, R. G., Erturk, N. & Heath, L. S. (2002). Role of superoxide dismutases in controlling oxidative stress in plants. Journal of Experimental Botany, 53(372), 1331-1341.
  4. Asada, K. (1999). The water- water cycle in chloroplast: scavenging of active oxygens and dissipation of excess photon. Annu. Rev. Plant Physiol. Plant. Journal of Molecular Biology, 50, 601-639.
  5. AzevedoNeto, A. D., PriSco, J. T., Eneas-Filho, J., Abreu, C. E. B. & Gomes-Filho, E. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt. Environ mental and Experimental Botany, 56, 81-94.
  6. Beers, G. R. & Sizer, I. V. (1952). Aspectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Biological Chemistry, 195, 133-140.
  7. Bittelli, M., Flury, M., Campbell, G. S. & Nichols, E. J. (2001). Reduction of transpiration through foliar application of chitosan. Agricultural and Forest Meteorology, 107, 167-175.
  8. Cavalcanti, F. R., Oliveira, J. T., Martins-Miranda, A., Viegas, A. S. & Silveira, R. A. (2004). Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt- saltstressed.
  9. Chakraborty, M., Karun, A. & Mitra, A. (2009). Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera. Journal of Plant Physiology, 166: 63-71.
  10. Chaves, M. M., J. P. Maroco and J. S. Pereira. 2003. Understanding plant responses to drought: From genes to the whole plant. Func. Plant Biology. Collingwood, 30(3), 239-264.
  11. Chen, W. P., Li, P. H. & Chen, T. H. H. (2000). Glycinbetaine increases chilling tolerance and reduces chilling induced lipid peroxidation in Zea mays L. Plant Cell Environment, 23, 609-618.
  12. Dat, J., Vandenabeele, S., Vranova, E., Van Montagu, M., Inze, D. & Van Breusegem, F. (2000). Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences57, 779-795.
  13. De Carvalho, M. H. C. (2008). Drought stress and reactive oxygen species. Plant Signal Behav, 3(3), 156-165.
  14. Del Rio, L. A., Sevilla, F., Sandalio, L. M. & Palma, J. M. L. (1991). Nutritional effects and expression of superoxide dismutase: induction and gene expression, diagnostics, prospective protection against oxygen toxicity. Free Radical Research. Communications. 12-13, 819-828.
  15. Guan, Y. J., Hu, J., Wang, X. J. & Shao, C. X. (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. J. Zhejiang Univ. Plant Science, 10, 427-433.
  16. Harish Prashanth, K. V., Dharmesh, S. M., Jagannatha Rao, K. S. & Tharanathan, R. N. (2007). Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage. Carbohydr. Research, 342, 190-195.
  17. Huang, R. H., Mendis, E. & Kim, S. K. (2005). Factors affecting the free radical scavenging behavior of chitosan sulfate. Journal of Biology, 36, 120-127.
  18. Hussain, A., Ghaudhry, M. R., Wajad, A., Ahmed, A., Rafiq, M., Ibrahim, M. & Goheer, A. R. (2004). Influence of water stress on growth, yield and radiation use efficiency of various wheat cultivars. Intrnational Journal of Agriculture and Biology, 6, 1074-1079.
  19. Jiang, H. F. & Ren, X. P. (2004). The effect on SOD activity and protein content in groundnut leaves by drought stress. Acra Agromomica Sinra, 30, 169-174.
  20. Jiang, M. & Zhang, J. (2001). Effect of abscisic acid on active oxygen species, antioxidative Defense system and oxidative damage in leaves of maize seedlings. Plant Cell Physiology, 42, 1265-1273.
  21. Khan, W., Prithiviraj, B. & Smith, D. L. (2003). Chitosan and chitinoligomers increase phenylalanine ammonia-lyaseand tyrosine ammonia-lyase activities in soybean leaves. Journal of Plant Physiology, 160, 859-63.
  22. Liu, Y., Cui, Y. & Mukherjee, A. (2007). Characterization of a novel RNA regulator of Erwinia carotovora spp. Carotovora that controls production of extracellular enzymes and secondary metabolites. Mol Microbiol, 29(1), 219-34.
  23. Mahdavi, B., Modarres Sanavy, S. A. M., Aghaalikhani, M. & Sharifi, M. (2011). Effect of water stress and chitosan on Germination and proline of seedling in safflower (Carthamus tinctorius L.). Journal of Crop Improvement, 25, 728-741.
  24. Mandal, S. (2010). Induction of phenolics, lignin and key defense enzymes in eggplant (Solanum melongena L.) roots in response to elicitors. Journal of Biotechnology, 9, 8038-8047.
  25. Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Science, 7, 405-409.
  26. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidases in spinach chloroplasts. Plant Cell Physiology, 22, 867-880.
  27. Park, P. J., Je, J. Y. & Kim, S. K. (2004). Free radical scavenging activities of differently deacetylated chitosans using an ESR spectrometer. Carbohydrate Polymers, 55, 17-22.
  28. Rinaudo, M. (2006). Chitin and chitosan: properties and application. Progress in Polymer Science, 31, 603-632.
  29. Scandalios, J. G. (1993). Oxygen stress and superoxide dismutase. Plant Physiology, 101, 7-12.
  30. Selote, D. S. & Khana-Chopra, R. (2004). Drought-induced spikelet sterility is associated with an inefficient antioxidant defence in rice plants. Physiologia Plantarum. 121, 462-467.
  31. Sharma, P. & Dubey, R. S. (2005). Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation, 46, 209-221.
  32. Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T. & Yabuta, Y. (2002). Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental Botany. 53, 1305-1319.
  33. Tan, Y., Liang, Z. S., Shao, H. B. & Du, F. (2006). Effect of water deficits on the activity of anti oxidative enzymes and osmoregulation among three different genotypes of Radix Astragali at seeding stage. Colloids and Surface Science B, 49, 60-65.
  34. Wajahatullah, Kh., Balakrishnan, P. & Donald, S. (2003). Chitosan and chitin oligomers increase phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities in soybean leaves. Journal of. Plant Physiology, 160, 859-863.
  35. Xie, W. M., Xu, P. X. & Liu, Q. (2001). Antioxidant activity of water-soluble chitosan derivatives. Bioorganic and Medicinal Chemistry Letters, 11, 1699-1701.
  36. Yen, M. T., Yang, J. H. & Mau, J. L. (2008). Antioxidant properties of chitosan from crab shells. Carbohydrate Polymers. 74, 840-844.
  37. Zhu, J. K. (2000). Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiology, 124, 941-948.