Effect of kaolin on the sunburn damage and qualitative characteristics of pomegranate fruit cv. Malas-e-Torsh-e-Saveh

Document Type : Full Paper

Authors

1 Assistant Professor, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

2 Associate Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran

Abstract

Sunburn damage is a physiological disorder, which reducing pomegranate fruits quality and result to huge loss to the gardeners yearly. In this study, the effects of kaolin on maintaining fruit quality and decreasing sunburn damage of Malas-e-Torsh-e-Saveh pomegranate was investigated. Processed Kaolin (surround® WP) with 0, 2.5, 5 and 10 % (w/v) was sprayed over the whole canopy and fruits at 45, 75 and 105 days after fruit set. The results indicated that kaolin significantly decreased fruit sunburn damage. Total soluble solid (TSS), total flavonoid content, total anthocyanin content and activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes reduced in kaolin treated fruits in compared to control. Furthermore, cyanidin 3-glucoside, cyanidin 3,5-diglucoside, delphinidin 3,5-diglucoside and pelargonidin 3,5-diglucoside content in kaolin treated fruits were lower than control. In contrast, fruit total phenolic content increased, but no significant difference was found for titratable acidity (TA), TSS/TA, pH, antioxidant activity and delphinidin 3-glucoside and pelargonidin 3-glucoside of pomegranate juice. Overall three times spraying with 5% kaolin concentration at 30 days intervals recommended for control of sunburn damage of pomegranate fruits.

Keywords


  1. Alighourchi, H., Barzegar, M. & Abbasi, S. (2008). Anthocyanins characterization of 15 Iranian pomegranate (Punica granatum L.) varieties and their variation after cold storage and pasteurization. European Food Research and Technology, 227, 881-887.
  2. Aly, M., El-Megeed, N. A. & Awad, R. M. (2010). Reflective particle films affected on, sunburn, yield, mineral composition and fruit maturity of ‘Anna’ apple (Malus domestica) trees. Research Journal of Agriculture and Biological Sciences, 6, 84-92.
  3. Beauchamp, C. & Fridovich, I. (1971). Superoxide dismutase: improved assays and applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287.
  4. Brand-Williams, W., Cuvelier, M. E. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Food Science and Technology, 28, 25-30.
  5. Cantore, V., Pace, B. & Albrizio, R. (2009). Kaolin-based particle film technology affects tomato physiology, yield and quality. Environmental and Experimental Botany, 66, 279-288.
  6. Chance, B. & Maehly, A.C. (1955). Assay of catalase and peroxidase. Methods in Enzymology, 2, 764-775.
  7. Ehteshami, S., Sarikhani, H. & Ershadi, A. (2011). Effect of kaolin and gibberellic acid application on some qualitative characteristics and reducing the sunburn in pomegranate Fruits (Punica granatum) cv. ‘Rabab Neiriz’. Plant Production Technology, 3, 15-24. (in Farsi)
  8. Gil, M.I., Tomas-Barberan, F.A. Hess-Pierce, B. Holcroft, D.M. & Kader, A.A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48, 4581-4589.
  9. Gindaba, J. & Wand, S. (2005). Comparative effects of evaporative cooling kaolin particle film and shade net on sunburn and fruit quality in apples. HortScience, 40, 592-596.
  10. Glenn, D. M., Prado, E. Erez, A. McFerson, J. & Puterka, G. J. (2002). A reflective, processed-kaolin particle film affects fruit temperature, radiation reflection, and solar injury in apple. Journal of the American Society for Horticultural Science, 127, 188-193.
  11. Glenn, D.M., Putreka, G.J. Drake, S.R. Unruh, T.R. Knight, A.L. Baherle, P. Prado, E. & Baugher, T.A. (2001). Particle film application influence apple leaf physiology, fruit yield, and fruit quality. Journal of the American Society for Horticultural Science, 126, 175-181.
  12. Guo, Y.P., Zhou, H.F. & Zhang, L.C. (2006). Photosynthetic characteristics and protective mechanisms against photooxidation during high temperature stress in two citrus species. Scientia Horticulturae, 108, 260-267.
  13. Hasanuzzaman, M., Nahar, K., Alam, M. Roychowdhury, R. & Fujita, M. (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. International Journal of Molecular Sciences, 14, 9643-9684.
  14. He, F., Mu, L., Yan, G. L., Liang, N. N., Pan, Q. H., Wang, J., Reeves, M. J. & Duan, C. Q. (2010). Biosynthesis of anthocyanins and their regulation in colored grapes. Molecules, 15, 9057-9091.
  15. Kulkarni, A. P. & Aradhya, S. M. (2005). Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93, 319-324.
  16. Meighani, H., Ghasemnezhad, M. & Bakhshi, D. (2014). Evaluation of biochemical composition and enzyme activities in browned arils of pomegranate fruits. International Journal of Horticultural Science and Technology, 1, 53-65.
  17. Melgarejo, P., Mrtinez, J. J., Hernandez, F., Martinez-Font, R., Barrows, P. & Erez, A. (2004). Kaolin treatment to reduce pomegranate sunburn. Scientia Horticulturae, 100, 349-353.
  18. Miguel, G., Fontes, C., Antunes, D., Neves, A. & Martins, D. (2004). Anthocyanin concentration of ‘Assaria’ pomegranate fruits during different cold storage conditions. Journal of Biomedicine and Biotechnology, 5, 338-342.
  19. Mousavinejad, G., Emam-Djomeh, Z., Rezaei, K. & Haddad-Khodaparast, M.H. (2009). Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars. Food Chemistry, 115, 1274-1278.
  20. Parchomchuk, P. & Meheriuk, M. (1996). Orchard cooling with pulsed over tree irrigation to prevent solar injury and improve fruit quality of Jonagold apples. HortScience, 31, 802-804.
  21. Singleton, V. L., Orthofer, R. & Lamuela-Raventós, R. S. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin- Ciocalteau Reagent. Methods in Enzymology, 299, 152-178.
  22. Song, J., Shellie, K.C. Wang, H. & Qian, M.C. (2012). Influence of deficit irrigation and kaolin particle film on grape composition and volatile compounds in Merlot grape (Vitis vinifera L.). Food Chemistry, 134, 841-850.
  23. Wand S. J. E., Theron, K. I., Ackerman, J. & Marais, S. J. S. (2006). Harvest and post-harvest apple fruit quality following applications of kaolin particle film in South African orchards. Scientia Horticulturae, 107, 271-276.
  24. Weerakkody, P., Jobling, J., Infante, M. & Rogers, G. (2010). The effect of maturity, sunburn and the application of sunscreens on the internal and external qualities of pomegranate fruit grown in Australia. Scientia Horticulturae, 124, 57-61.
  25. Yazici, K. & Kaynak, L. (2009a). Effects of kaolin and shading treatment on sunburn on fruit of Hicnazar cultivar of pomegranate (Punica granatum L. cv. Hicnazar). Acta Horticulturae, 818, 167-174.
  26. Yazici, K. & Kaynak, L. (2009b). Effects of air temperature, relative humidity and solar radiation on fruit surface temperatures and sunburn damage in pomegranate (Punica granatum L. cv. Hicaznar). Acta Horticulturae, 818, 181-186.