Evaluation of Satureja khuzistanica micropropagation as a medicinal plant

Document Type : Full Paper

Authors

1 . Assistant Professor, Department of Horticulture, Faculty of Agriculture, Lorestan University, Khorram Abad, Iran

2 . Assistant Professor, Department of Horticulture, Faculty of Agriculture, University of Guilan, Rasht, Iran

Abstract

An experiment was conducted to study the micropropagation of Satureja khuzistanica. The seeds were planted in 1/2MS medium. After germination and growth of plantlets, they were subcultured in fresh medium. Then, they cultured into medium supplemented by different concentrations of BA and Kinetin. Results showed that, the highest proliferation (2.4 shoots) was observed at concentrations of 0.5 mg/l IBA and 3 mg/l BA while the lowest proliferation (1.12 and 1.04 shoots) was produced at concentration of 0.25 mg/l IBA, 1.5 mg/l BA and also 0.5 mg/l Kinetin. The tallest shoots (5.76 cm) were obtained at concentration of 0.5 mg/l IBA and 1.5 mg/l BA whereas the smallest ones (2.44 cm) were observed at concentration of 1 mg/l Kinetin. Lowest percentage of survived plantlets (46.6 %) were observed in the media with IBA at concentration of 0.25 mg/l and BA at concentration of 1.5 mg/l. Quarter salt strength MS basal medium induced rich rooting response (85.2%), when compared with ½ salt strength MS (46.66%) and MS (17.6%), media. The highest rooting percentage (64.30%) and number of roots (7.7 and 8. 4) were obtained at 0.25 and 0.50 mg/l IBA, respectively. Overall, media containing 0.25 mg/l IBA plus 3 mg/l BA can be recommended for micropropagation of S. khuzistanica.
 

Keywords

References

  1.  

    1. Afzalifar, M. (2012). Evaluation of different propagation and androgenesis in Satureja khuzistanica and S. rechingeri. M.Sc. thesis. Medicinal Plants and Drug Research Institute. Shahid Beheshti University, Iran. (in Farsi)
    2. Arikat, N. A., Jawad, F. M., Karam, N. S. & Shibli, R. A. (2004). Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill.). Scientia Horticulturae, 100, 193-202.
    3. Cristna, M. F. D., Esquibel, M. A. & Dos Santos, A.V. P. (1990). In vitro propagation of the alkaloid producing plant Datura insignis Barb. Rodr. Plant Cell Tissue and Organ Culture, 21, 75-78.
    4. Cuenca, S. & Amo-Marco, J. B. (2000). In vitro propagation of two Spanish endemic species of Salvia through bud proliferation. In Vitro Cellular & Developmental Biology – Plant, 36, 225-229.
    5. Fotovati, H. & Noorbakhsh, S. (2009). Biotechnology, the new aspect for agricultural progress. The 6th National Biotechnology Congress of Iran, Tehran. (in Farsi).
    6. Hadian, J. (2008). Evaluation of genetic diversity of endemic Iranian Satureja species. PhD thesis. University College of Agriculture and Natural Resources, University of Tehran, Iran.(in Farsi)
    7. Hadian, J., Mirjalili, M. H. & Ganjipoor, N. (2011). Morphological and phytochemical characterization of natural populations of Satureja khuzestanica. Chemistry & Biodiversity, 8, 902-915.
    8. Iram, S. & Anis, M. (2008). An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiologiae Plantarum, 30, 493-499.
    9. Jamzad, Z. (1994). A new species of the genus Satureja from Iran. The Iranian Journal of Botny, 6(2), 215-218.
    10. Mao, A. H., Wettem, A., Fay, M. & Caligari, P. D. S. (1995). In vitro propagation of Clerodendrum colebrookianum Walp, a potential natural anti-hypertension medicinal plant. Plant Cell Reports, 14, 493-496.
    11. Morimoto, S., Goto,Y. & Shorama, Y. (1994). Production of lithospermic acid B and rosmarinic acid in callus tissue and regenerated plantlets of Salvia miltiorrhiza. Journal of Natural Products, 57, 817-823.
    12. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiology, 15, 473-497.
    13. Pereira, A. M. S., Bertoni, B. W., Moraes, R. M. & Franca, S. C. (2000). Micropropagation of Salix humboldtiana Hild. Revista Brasileriade Plantas Medicinasis, 2, 17-21.
    14. Preira, N. A. B. (1996). In vitro propagation of Hancornia speciosa, a tropical fruit tree. In Vitro Cellular Development Biology, 32, 253-256.
    15. Saxena, C., Rout, G. R. & Das, P. (1998). Micropropagation of Psoralea corylifolia.Linn. Journal of Medicinal and Aromatic Plant Sciences, 20, 8-15.
    16. Sefidkon, F., Abbasi, K., Jamzad, Z. & Ahmadi, S. (2007). The effect of distillation methods and stage of plant growth on the essential oil content and composition of Satureja rechingeri Jamzad. Food Chemistry, 100 (3), 1054-1058.
    17. Tripathi, L. & Tripathi, J. N. (2003). Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research, 2, 243-253.

     

Iranian Journal of Horticultural Science
Volume 46, Issue 2 - Serial Number 2
August 2015
Pages 291-296

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History

  • Receive Date: 02 December 2013
  • Revise Date: 23 May 2014
  • Accept Date: 13 July 2014

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