Introduction of commercial protocol for in vitro proliferation of Brassica oleracea var. acephala

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Abstract

In recent years, ornamental kale is commonly used in landscapes due to its colorful shoots and resistance to the diverce environmental conditions. Thus, its in vitro proliferation was studied in series of experiments. Different BA (0/10.1, 0.5 and 1 mg/L), 2,4-D (0.1, 0.5 mg/L) and silver nitrate (2, 4 mg/L) treatments were applied for the establishment stage. Treatments of the proliferation stage also included BA (0.1 and 0.5 mg/L) and IBA (0.1 and 0.5 mg/L). Acclimatization of in vitro plantlets was conducted using different concentrations of MS (complete and half strength) and vermicompost extract (direct and diluted). Results revealed that, using of 0.1 mg/L BA with one mg/L 2,4-D was able to provide regeneration in this ornamental plant, acceptably. Proliferation medium supplemented with 0.5 and 0.5 mg/L BA caused shoot development. Finally, in vitro plantlets were successfully adapted by transferring to media containing reduced MS medium without plant growth regulators. In conclusion, by using the relatively low cost, accessible media and the least concentrations of plant growth regulators, successful regeneration of kale ornamental were obtained.

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References

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History

  • Receive Date: 23 November 2013
  • Revise Date: 22 January 2014
  • Accept Date: 01 February 2014

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