Investigation of Genetic Diversity and Relationships of ‘Kurdistan’ Strawberry to some Commercial Cultivars using Microsatellite Markers

Lotfi, Abbas; Gharaghani, Ali; Tafazoli, Enayatoolah; Niazi, Ali; Eshghi, Saeed; Mozafari, Ali-Akbar

doi:10.22059/ijhs.2014.50940

Investigation of Genetic Diversity and Relationships of ‘Kurdistan’ Strawberry to some Commercial Cultivars using Microsatellite Markers

Document Type : Full Paper

Authors

Abbas Lotfi ¹

Ali Gharaghani ²

Enayatoolah Tafazoli ³

Ali Niazi ⁴

Saeed Eshghi ⁵

Ali-Akbar Mozafari ⁶

¹ MSc., College of Agriculture, University of Shiraz, Shiraz, Iran

² Assistant Professor, College of Agriculture, University of Shiraz, Shiraz, Iran

³ Professor, College of Agriculture, University of Shiraz, Shiraz, Iran

⁴ Associate Professor, Institute of Biotechnology, University of Shiraz, Shiraz, Iran

⁵ Associate Professor, College of Agriculture, University of Shiraz, Shiraz, Iran

⁶ Assistant Professor, College of Agriculture, Kurdistan University, Kurdistan, Iran

10.22059/ijhs.2014.50940

Abstract

In order to evaluate genetic relationship, diversity and possible genetically and
physical mixing of Kurdistan cultivar with Russian, American and European cultivars a
collection of 33 samples including: 20 accessions of cv. Kurdistan from different fields
of Kurdistan province, 7 Russian and 6 American and European cultivars were analyzed
using 10 molecular markers. Result indicated that in each microsatellite locus, between
4 to 8 (with an average of 5.5) alleles were seen and Polymorphism information content
of loci varied between 0.59- 0.86. Using cluster and also factor analysis, cultivars were
divided in to 8 groups: (including 2 main groups of Kurdistan cultivar samples and
Russian cultivars, the rest of the commercial cultivars were placed in the 4th to 8th
groups. High Similarity was obtained between Kurdistan cultivar and Russian Cultivars.
In comparison to American and European cultivars, considering historical background,
it could mean that Kurdistan variety might have been a Russian cultivar originally,
which has brought to Iran in the past. Results of this study revealed some cases of
physical mixing of Kurdistan strawberry with other commercial cultivars in growing
fields and also a case of mutation of this cultivar.

Keywords

Kurdistan strawberry

Genetic relationship

genetic mixing

microsatellite marker

commercial strawberry cultivars