Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effects of aminolevulinic acid on growth, antioxidant activity and anthocyanin accumulation of apple explants under salinity stress in in vitro culture conditions

Document Type : Full Paper

Authors
Fatemeh Zahedzadeh 1
Fariborz Zaare Nahandi 2
Mohammad Reza Dadpour 2
Ali Reza Motallebi-Azar 2
Saiedeh Alizadeh Salteh 3
1 Former Ph.D. Student, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Assistant Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
10.22059/ijhs.2018.255705.1437
Abstract
The present study was carried out to evaluate the effect of 5-aminolevulinic acid (ALA) on physiological and biochemical changes of in vitro-cultured Budaguvsky 9 (Bud 9) apple cultivar under NaCl induced salinity stress. Virus-free shoots and callus  of Bud 9 apple  were cultured on MS medium containing different levels of NaCl (0, 30, 60 and 90 mM) and ALA (0, 2.5, 5, 10 and 20 µM). Four weeks later phytochemical variations of regenerated plantlets with or without NaCl and/or ALA treatments were recorded.  Shoot explants and callus treated with 5-aminolevolinic acid at 2.5 to 20 µM concentrations demonstrated reduction in catalase and peroxidase and enhancement in superoxide dismutase and ascorbate peroxidase enzyme activity. The highest chlorophyll a and b values were observed at shoot explants treated with concentration of 10 μm of aminolevolinic acid. Also, salinity stress was performed an effective role on increasing the amount of anthocyanins of explants and the highest amount was obtained in 90 mM salinity stress. Increasing the intensity of salinity stress and amino acid concentration (2.5-10 μm) in culture media had synergic effect on the antioxidant activity, total phenol as well as flavonoids content of studied explants.  
Keywords
Antioxidant Enzymes
biochemical change
Bud 9 apple
callus
explant
Subjects
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Iranian Journal of Horticultural Science
Volume 50, Issue 3
Autumn 2019
Pages 667-681

  • Receive Date 22 April 2018
  • Revise Date 20 August 2018
  • Accept Date 01 September 2018