Association analysis of morphological traits in tomato using ISSR markers

Document Type : Full Paper


1 Member of the Scientific Board, Seed and Plant Improvement Research Department, West Azarbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Urmia, Iran

2 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia

3 Professor, Department of Horticulture, Faculty of Agriculture, Ataturk University of Turkey, Erzurum, Turkey


Tomato is one of the most economically important vegetable crops in many parts of the world. Improvement of yield and other essential characteristics of tomato have received more attention. Mapping quantitative trait loci could accelerate tomato breeding processes. ISSR markers were used to identify molecular makers associated with qualitative and quantitative traits in 93 landraces (from West Azarbaijan in Iran and Iğdır in Turkey) and three commercial cultivars. Association analysis using mixed linear model (MLM) identified 74 ISSR markers significantly (p<0.05) associated with 12 qualitative traits. The highest and lowest number of associated markers achieved for fruit size homogeneity (4 markers) and depression at peduncle end (10 markers), respectively. For quantitative traits, 93 ISSR markers were found to be significantly (p<0.02) associated with 20 traits. The highest and lowest number of associated markers was observed for cotyledon leaf width (one marker) and fruit length and fruit pH (9 markers), respectively. Some markers showed significant association with a number of quantitative traits in both years. Also, siginificant associations were found between a marker and various traits. After validation of these markers, we will be able to use them in tomato breeding programs for simoultaneous mutil-trait selection. 


Main Subjects

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