Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

The effect of foliar application of iron on some biochemical and photosynthetic characteristics of Holy basil (Ocimum sanctum)

Document Type : Full Paper

Authors
Mohammad Mahmoodi Sourestani 1
Emel Moghadam 2
Ahmad Farrokhian Firoozi 1
1 Assistant Professor, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 M.Sc. Student, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.22059/ijhs.2016.59816
Abstract
Holy basil (Ocimum sanctum) is one of the important medicinal plant belongs to Lamiaceae family. In order to evaluate the effect of foliar application of iron on iron and chlorophyll contents and photosynthetic parameters of holy basil, an experiment was conducted at research farm of Department of Horticultural Science, Shahid Chamran University of Ahvaz based on randomized complete block design, with six treatments and three replications. The treatments were control, nano iron chelate (0.5, 1 and 1.5 g.l-1) and iron chelate (1 and 1.5 g.l-1) fertilizers. The results showed that the effect of foliar application of iron fertilizers was significant on iron and chlorophyll contents, stomatal conductance, transpiration, net photosynthesis and quantum yield. The highest iron content, stomatal conductance, transpiration and quantum yield were observed in plant sprayed with 1 g.l-1 nano iron chelate. The highest values of chlorophyll, cartenoid and net photosynthesis were obtained in plant sprayed with 1.5 g.l-1 nano iron chelate. The lowest values of mentioned traits were observed in control treatment. Eugenol, 1,8-cineol and methyl chavicol were  the main oil components. Overall, in regard to obtained results, for increasing mentioned traits of holy basil, foliar application of nano iron chelate with 1 g.l-1 is recommended.
Keywords
Chlorophyll
Holy basil
iron
Net photosynthesis
  1. Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112- 121.
  2. Balakrishnan, K., Rajendran, C. & Kulandaivelu, G.  (2000). Differential responses of iron, magnesium, and zinc deficiency on pigment composition, nutrient content and photosynthetic activity in tropical fruit crops. Photosynthetica, 38, 477- 479.
  3. Gang, D. R., Wang, J., Dudareva, N.,  Nam, K. H., Simon, J. E., Lewinsohn, E. & Pichersky, E., 2001. An investigation of the storage and biosynthesis of phenylpropenes in sweet basil. Journal of Plant Physiology, 125, 539- 555.
  4. Harsa, B.H., Hebbar, S.S., Shripathi, V. & Hedge, G.R. (2003). Ethnobotany of Uttara Kannada district in Karnataka, India - plants in treatment in skin diseases. Journal of Ethnopharmacology, 84, 37-40.
  5. Kakaraparthi, P.S., Rajput, D.K. & Arigarui, N.K. (2013). Response of Ocimum tenuiflorum variety CIM-AYU to sulphur fertilization in the semi-arid tropical region of Deccan plateau in India. International Journal of Scientific Research, 2(10), 1- 6.
  6. Kalyankumar, P., Rupesh Kumar, M., Kavitha, K., singh, J. & Rawoof, Kh. (2012). Pharmacological Actions of Ocimumsacntum-Review Article. International Journal of Advances in Pharmacy, Biology and Chemistry, 1(3), 406- 411.
  7. Khoshgoftarmanesh, A.H.  (2007). Principle in plant nutrition. Isfahan University of Technology Pub. (in Farsi)
  8. Kiani, Sh. (2012). Effects of iron on efficiency and map of photosystem II photochemical yield of rose flower using chlorophyll fluorescence imaging. Journal of Science and Technology of Greenhouse Culture, 2(8), 25-35. (in Farsi)
  9. Kibler, A. (2014). Establishment of an efficient protocol for the Micro propagation of Holy Basil (Ocimum Sanctum L.). M.Sc. thesis, Faculty of Agriculture. Guelph University, Canada.
  10. Larbi, A., Morales, F., Lopez-Millan, A. F., Gogorcena, Y., Abadia, A., Moog, P. R. & Abadia, J. (2001). Technical advance: reduction of Fe (III) chelate by mesophyll leaf disks of sugar beet. Multi- component origin and effect of Fe deficiency. Plant Cell Physiology, 42(1), 94- 105.
  11. Lindsay, W.L. & Norvell, W.A. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America, 42, 421-428.
  12. Mahmoodi, M. (2013). Diurnal variations of gas exchange characteristics in leaves of anise hyssop (Agastache foeniculum) under normal, drought stress and recovery conditions. Journal of Medicinal Plants and By-products, 1, 91-101.
  13. Marscher, H. (2011). Mineral Nutrition of High Plant. (3th ed.). Academic press.
  14. Mazaherinia, S., Astaraei, A., Fotovat, A. & Monshi, A. (2010).  Effect of iron oxides (ordinary and nano) and municipal solid waste compost (MSWC) coated sulfur on wheat (Triticum aestivum L.) plant iron concentration and growth, Journal of Iranian Field Crop Research, 8(5), 855-861. (in Farsi)
  15. Misra, A., Dwivedi, S., Tewari, S., Khan, A. & Kumar, R. (2007). Analysis of growth, physiology, photosynthesis, essential monoterpene oil (s) yield and quality in Ocimum sanctum L. genotypes. Bioscience Research, 4(1), 1-5.
  16. Mohammadi poor, R., Sedaghathoor, S.  & Mahboub homami, A. (2013). Effect of application of iron fertilizers in two methods 'foliar and soil application' on growth characteristics of Spathyphyllum illusion. European Journal of Experimental Biology, 3(1), 232- 240.
  17. Mondal, Sh., Mirdha, B.R. & Mahapatra, S.C. (2009). The science behind sacredness of tulsi(Ocimum sanctum L.). Indian Journal of Physiology and Pharmacology, 53(4), 291-306.
  18. Morales, F., Abadía, A. & Abadía, J. (1998). Photosynthesis, quenching of chlorophyll fluorescence and thermal energy dissipation in iron-deficient sugar beet leaves. Journal of Plant Physiology, 25, 403-412.
  19. Mousa, G.T., El-Sallami, I. H. & Ali, E. F. (2003). Response of Nigella sativa L. to foliar application of gibberellic acid, benzyl adenine, iron and zinc. Assiut Journal of Agricultural of Science (Egypt), 32, 141-156.
  20. Nasiri, Y., Zehtab Salmasi, S., Nasrullah Zadeh, S., Ghassemi Gholezani, K., Najafi, N. & Javanmard, A. (2013). Evaluation of foliar spray of ferrous sulfate and zinc sulfate on yield and nutrients concentration of aerial parts in German chamomile.  Journal of Agricultural Science, 23(3), 105-115. (in Farsi)
  21. Peyvandi, M., Mirza, M. & Kamali Jamakani, Z. (2011). The effect of nano Fe chelate and Fe chelate on the growth and activity of some antioxidant of Satureja hortensis, New Cellular and Molecular Biotechnology Journal, 2(5), 25-32. (in Farsi)
  22. Peyvandi, M., Parande, H. & Mirza, M. (2011). Comparison of nano Fe chelate with Fe chelate effect on growth parameters and antioxidant enzymes activity of Ocimum basilicum.New Cellular and Molecular Biotechnology Journal, 1(4), 89-98. (in Farsi)
  23. Pojjanapimol, S. (2004). Characterization of aroma impact compounds in fresh, heated and dried holy basil (Ocimum sanctum) Leaves. Ph.D. Dissertation, Kasetsart University, Thailand.
  24. Prakash, P. & Gupta, N. (2005). Therapeutic uses of Ocimum sanctum Linn (tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian Journal of Physiology and Pharmacology, 49(2), 125-131.
  25. Rombol, A.D., Gogorcena, Y., Larbi, A., Morales, F., Baldi, E., Marangoni, B., Tagliavini, M. & Abad, J. (2005). Iron deficiency-induced changes in carbon fixation and leaf elemental composition of sugar beet (Beta vulgaris) plants. Plant and Soil, 271, 39-45.
  26. Sharma, T. (2010).Toxic effect of Ocimum sanctum plant extract against Acrida exaltata (Orthoptera: Acrididae) adults.  Journal of Environmental Research and Development, 4(4), 1008-1012.
Iranian Journal of Horticultural Science
Volume 47, Issue 3 - Serial Number 3
Autumn 2016
Pages 543-552

  • Receive Date 16 February 2015
  • Revise Date 10 April 2015
  • Accept Date 19 May 2015