Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Evaluation of some the physiological and growth responses of three walnut genotypes to different bicarbonate concentrations in irrigation water

Document Type : Full Paper

Authors
Meysam Manzari Tavakkoli 1
Vahed Bagheri 2
Hamid Reza Karimi 3
Hamid Reza Roosta 3
1 Former M.Sc. Student, Department of Horticultural Sciences, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran
2 Former Ph.D. Student, Department of Horticultural Sciences, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran
3 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Iran
10.22059/ijhs.2015.56904
Abstract
In order to evaluate the effects of sodium bicarbonate of irrigation water on the physiology and growth characteristics ofwalnut genotypes, a factorial experiment was performed with two factors including bicarbonate at 3 levels (0, 20 and 40 mM) and walnut genotypes (including local genotype A and wild genotypes B and C), based on completely random design. In this study, the effect of bicarbonate on various traits such as growth, photosynthetic parameters, osmotic adjustment compounds and nutrient elements of each genotype were examined. Results showed that bicarbonate affected all traits, and the differences among the three genotypes were clearly obvious. Irrespective of genotype, iron concentration decreased in shoots of all plants. The lowest reduction (15.17%) of iron concentration of shoots at 40 mM bicarbonate was related to the genotype A. The lowest chlorophyll percentage was observed in genotype A when exposed to bicarbonate, as compared with control. Results showed that walnut belongs to the group of horticultural crops that are highly sensitive to bicarbonate existance in irrigation water. So that, the growth of plants began to reduce from 20 mM treatment. According to the results of this study, genotype A had the highest tolerance to bicarbonate and had better growth in these conditions. Although, there was no significant difference between genotype B and C. Therefore, genotype A can be introduced as a tolerant genotype to bicarbonate existence in irrigation water and can be used in walnut breeding programs.  
Keywords
alkalinity
iron
pH
tolerant genotypes
Walnut
  1. Ahmad, P. & Sharma, S. (2010). Physio-biochemical attributes in two cultivars of mulberry (Morusalba L.) under NaHCO3 stress. International Journal of Plant Production, 4 (2), 1735-6814.
  2. Alcantara E., Manuel Cordeiro A. & Barranco D. (2003). Selection of olive varieties tolerance to iron chlorosis. Plant Physiology, 160, 1467-1477.
    1. Alcantara, E., Romera, F. J. & de la Guardia, M. D. (1988). Genotypic differences in bicarbonate-induced iron chlorosis in sunflower. Journal of Plant Nutrition, 11, 65-67.
    2. Alhendawi, R. A., Römheld, V. E., Kirkby, A. & Marschner, H. (1997). Influence of increasing bicarbonate concentrations on plant growth, organic acid accumulation in roots and iron uptake by barley, sorghum and maize. Journal of Plant Nutrition, 20, 1731-1753.
    3. Barber, S. A. (1995). Soil Nutrient Bioavailability, A Mechanistic Approach. (2nd ed.). John Wiley & Sons, New York.
    4. Bavaresco, L., Giachino, E. & Colla, R. (1999). Iron chlorosis paradox in grapevine. Journal of Plant Nutrition, 22, 1589-1597.
    5. Bertoni, G. M., Pissaloux, A., Morad, P. & Sayag, D. R. (1992). Bicarbonate-pH relationship with iron chlorosis in white lupine. Journal of Plant Nutrition, 15, 1509- 1518.
    6. Bialczyk, J., Lechowski, Z. & Libik, A. (1994).Growth of tomato seedlings under different HCO3- concentration in the medium. Journal of Plant Nutrition, 17, 801-816.
    7. Bugbee, B. (2003). Nutrient management in recirculating hydroponic culture. Available at http://www.usu.edu/cpl/research hydroponics 3. htm. Accessed 22 November 2010.
    8. Chapman, H. D. & Pratt, P. F. (1982). Methods of Analysis for Soils, Plants and Waters. Division of Agriculture, University of California, Berkeley, CA. 4034p.
      1. Colla, G., Rouphael, Y., Cardarelli, M., Salerno, A. & Rea, E. (2010). The effectiveness of grafting to improve alkalinity tolerance in watermelon. Environmental & Experimental Botany, 68,283-291.
      2. Darvishyan, M. (1997). Walnut culture to new method. Publication company technical of Iran. (in Farsi)
        1. De la Guardia, M. D. & Alcántara, E. (2002). Bicarbonate and low iron level increase root to total plant weight ratio in olive and peach rootstock. Journal of Plant Nutrition, 25, 1021-1032.
        2. Deng, C. N., Zhang, G. X., Pan, X. L. & Zhao, K. Y. (2010). Chlorophyll fluorescence and gas exchange responses of maize seedlings to saline-alkaline stress. Bulgarian Journal of Agricultural Science, 16(1), 49-58.
        3. Fernandez, V., Ebert, G. & Winkelmann, G. (2005). The Use of microbial siderophores for foliar iron application studies. Plant & Soil, 272, 245-252.
        4. Hakam‚ P., khanizade‚ S., Deell‚ J. R. & Richr‚ C. (2000). Assessing chilling tolerance in roses using chlorophyll fluorescence. HortScience, 35, 184-186.
          1. Irigoyen, J. J., Emerich, D. W. & Sanchez- Diaz, M. 1992. Water stress induced changesin concentrations of proline and total soluble sugars in nodulated alfalfa (medicago sativa) plants. Physiologia Plantarum, 84, 67-72.
          2. Ksouri, R., Debez, A., Mahmoudi, H., Ouerghi, Z., Gharsalli, M. & Lachaal, M. (2007). Genotypic variability within Tunisian grapevine varieties (Vitis vinifera L.) facing bicarbonate-induced iron deficiency. Plant Physiology & Biochemistery, 45, 315-322.
          3. Lichtenthaler, H. K. (1987). Chlorophylls and Cartenoids: Pigments and Photosynthetic Biomembranes. Methods in Enzymology, 148, 350-382.
          4. Liu, J. & Shi, D. C. (2010). Photosynthesis, chlorophyll fluorescence, inorganic ion and organic acid accumulations of sunflower in responses to salt and salt-alkaline mixed stress. Photosynthetica, 48, 127-134.
          5. Malassiotis, A., Tanou, G., Diamantidis, G., Patakas, A. & Therios, L. (2006). Effects of 4-month Fe deficiency exposure on Fe reduction mechanism, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant defense in two peach rootstocks differing in Fe deficiency tolerance. Journal of Plant Physiology, 163, 176-185.
            1. Marschner, H. (1995). Mineral Nutrition of Higher Plants. IIthedn. Press, London.
            2. Nikolic, M. & Kastori, R. (2000). Effect of bicarbonate and Fe supply on Fe nutrition of grapevine. Journal of Plant Nutrition, 23, 1619-1627.
              1. Paquin R. & Lechasseur, P. (1979). Observations sur une methode dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany, 57, 1851-1854.
              2. Peng, Y. L., Gao, Z. W., Gao, Y., Liu, G. F., Sheng, L. X. & Wang, D. L. (2007). Ecophysiological characteristics of alfalfa seedling in response to various mixed salt-alkaline stresses. Horticultural Science, 58, 240-278.
                1. Pissaloux, A., Morarad, P. & Bertoni, G. (1995). Alkalinity-Bicarbonate Calcium Effects on Iron Chlorosis in White Lupine in Soilless Culture. In: Abadia, J. (ed.). Development in plant and soil science. Iron nutrition in soils and plants; Seventh International Symposium on Iron Nutrition and Interactions in Plants, vol. 59. Zaragoza, Spain, June 27–July 2, 1993. Kluwer Academic Publishers, Dordrecht, 127-133.
                2. 27.           Romanowska-Duda, B., Kalaji, M. H. & Strasser, R. J. (2005). The Use of PSII Activity of Spirodela Oligorrhiza Plants as an Indicator for Water Toxicity. In: Van der Est A., Bruce D. Photosynthesis: Fundamental Aspects to Global Perspectives. Allen Press, Lawrence.
                  1. Römheld, V. (2000). The chlorosis paradox: Fe inactivation as a secondary event in chlorotic leaves of grapevine. Journal of Plant Nutrition, 23, 1629-1643.
                  2. Roosta, H. R. & J. K. Schjoerring. (2007). Effects of ammonium toxicity on nitrogen metabolism and elemental profile of cucumber (Cucumis sativus L., cv. Styx) plants. Journal of Plant Nutrition, 30, 1933-1951.
                  3. Satoh, R., Nakashima, K., Seki, M., Shinozaki, K. & Yamaguchi-Shinozaki K. (2002). ACTCAT, a novel cis-acting element for proline- and hypoosmolarity-responsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis. Plant Physiology, 130, 709-719.
                  4. Shahabi, A., Malakouti, M. J. & Fallahi, E. (2005). Effects of Bicarbonate content of irrigation water on nutrition disorders of some apple varieties. Journal of Plant Nutrition, 28, 1663-1678.
                  5. Shi, D. C. & Yin, L. J. (1993). Different between salt (NaCl) and alkaline (Na2CO3) stresses on Puccinellia tenuiflora (Griseb) Scribn. et. Merr. Plants. Journal of Integrative Plant Biology,35, 144-149.
                  6. Shi, D. C. & Zhao, K. F. (1997). Effects of NaCl and Na2CO3 on growth of Puccinellia tenuiflora and on present state of mineral elements in nutrient solution. Acta Prataculturae Sinica, 6, 51-61.
                  7. Shi, Y., Byme, D. H., Reed, D. W. & Loeppert, R. H. (1993). Iron chlorosis development and growth response of peach rootstocks to bicarbonate. Journal of Plant Nutrition, 16, 1039-1046.
                  8. Singleton, V. L. & J. Rossi, A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology & Viticulture, 16, 144-153.
                  9. Soltani, A. (2004). Chlorophyll Fluorescence and Its Application. Internal Press. University of Agricultural Science and Natural Resource, Gorgan, Iran. (in Farsi)
                    1. Strasser, R. J., Srivastava, A. & Tsimilli-Michael, M. (2000). The Fluorescence Transient as Tool to Characterize and Screen Photosynthetic Samples. In: Yunus, M., Pathre, U., Mohanty, P. Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Taylor and Francis, London.
                    2. Taiz, L. & Zeiger, E. (2002). Plant Physiology. (3nd Edition). Sinauer Assoc. Inc., Publishers, Sunderl and MA, pp: 283- 308.
                    3. Valdez-Aguilar, L. A. & Reed, D. W. (2010). Growth and nutrition of young bean plants under high alkalinity as affected by mixtures of ammonium, potassium, and sodium. Journal of Plant Nutrition, 33, 1472-1488.
                      1. Valdez-Aguilar, L. A. (2004). Effect of alkalinity in irrigation water on selected greenhouse ornamental plants. Ph.D Dissertation, College Station, Texas: Texas A&M University.
                      2. Wang, X., Geng, S., Ri, Y. J., Cao, D., Liu, J., Shi, D. C. & Yang, C. W. (2011). Physiological responses and adaptive strategies of tomato plants to salt and alkali stresses. Scientia Horticulturae, 130, 248-255.
                      3. Whipker, B. E., Bailey, D. A.,  Nelson, P. V.,  Fonteno, W. C, & Hammer, P. A. 1996. Anoval approach calculate acid additions for alkalinity control in grrenhouse irrigation water. Communications in Soil Science and Plant Analysis, 27, 959-976.
                      4. Yang, C. W., Xu, H. H., Wang, L. L., Liu, J., Shi, D. C. & Wang, D. L. (2009). Comparative effects of salt-stress and alkali-stress on the growth, photosynthesis, solute accumulation, and ion balance of barley plants. Photosynthetica, 47, 79-86.
                      5. Yang, J. Y., Zheng, W., Tian, Y., Wu, Y. & Zhou, D. W. (2011). Effects of various mixed salt-alkaline stresses on growth, photosynthesis, and photosynthetic pigment concentrations of Medicago ruthenica seedlings. Photosynthetica, 49, 275- 284.
                        1. Yang, X., Römheld, V. & Marschner, H. (1993). Effect of bicarbonate and root zone temperature on uptake of Zn, Fe, Mn and Cu by different rice cultivars (Oryza sativa L.) grown in calcareous soil. Plant & Soil, 155, 441-444.
                        2. Zribi, K. & Gharsalli, M. (2002). Effect of bicarbonate on growth and iron nutrition of pea. Journal of Plant Nutrition, 25, 2143-2149.
Iranian Journal of Horticultural Science
Volume 46, Issue 4 - Serial Number 4
Winter 2016
Pages 549-561

  • Receive Date 07 November 2013
  • Revise Date 30 April 2014
  • Accept Date 23 August 2014