Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Quantitative expression analysis of CBF1 and CBF4 genes under cold stress treatments in grape cultivars “Khalili-Danedar”, “Shahroodi” in comparison with Vitis Riparia

Document Type : Full Paper

Authors
Ali Ebadi 1
Maryam Karimi Alvijeh 2
SeyedAmir Moosavi 3
Seyed Alireza Salami 4
1 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Post Graduate Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Genetic Engineering Research Institute of Iran
4 Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijhs.2015.55859
Abstract
With regard to its economic importance of grapevine, study on different aspects of factors that induce hardiness to the unexpected early-season and late-season cold seems to be essential. Some of the transcription factors are essential for synthesis of proteins that are important for cold adaptation. In the present study, differences in the expression patterns of two CBF1 and CBF4 transcription factors were evaluated under cold stress conditions in the “Khalili-Danedar”, “Shahroodi” and “Riparia”. Results showed that expression of CBF1 was increased at early minutes of cold stress and “Riparia” showed higher expression compared with two other genotypes. Also the least expression was recorded for “Shahroodi” after 24 h of cold treatment. Regarding CBF4, increase in the expression was started one hour after cold treatment and similar to the CBF1, the highest expression was recorded for “Riparia”. Results of expression patterns of these two genes in “Riparia” grape showed that expression of CBF4 was about 10 fold of CBF1.
Keywords
grape
transcription factors
Cold stress
gene expression
CBF1
CBF4

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Iranian Journal of Horticultural Science
Volume 46, Issue 3 - Serial Number 3
Autumn 2015
Pages 379-386

  • Receive Date 24 February 2014
  • Revise Date 30 June 2014
  • Accept Date 17 August 2014