Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Evaluation of genetic diversity of some Iranian edible Crocus species using morphological and ISSR marker

Document Type : Full Paper

Authors
Bita Khansarinejad 1
Mohamad Reza Hasandokht 2
Vahide Nazeri 2
Aboozar Soorni 2
1 , Former Graduate Student, Associate Professor, Associate Professor and Ph.D Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Former Graduate Student, Associate Professor, Associate Professor and Ph.D Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijhs.2015.54124
Abstract
Abstract
In this study, two wild Iranian Crocus species (Crocus speciosus and Crocus cancellathus) were examined using 16 morphological characters and 13 ISSR primers on 250 and 25 plant samples, respectively. The highest and lowest coefficient of variations were belonged to the thickness of the petals (71.25%) and the leaf length (15.08%), respectively. Cluster analysis based on morphological characteristics divided plant samples into three groups. Thirteen ISSR primers produced high degree of polymorphism (94.46%). Genetic similarities among the samples ranged from 0.11 to 0.75 for Khanemiran–Arak with HassanAbad-shazand (C. cancellathus) and for Ravansar with-Kangavar (C. speciosus), respectively. Results showed that the plant samples belonging to two studied species, had high genetic diversity and ISSR analysis was suitable technique for evaluation of genetic diversity of Crocus species.
Keywords
Cluster Analysis
Crocus
ISSR marker
Morphological characters

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  • Receive Date 22 December 2013
  • Revise Date 02 June 2014
  • Accept Date 14 June 2014