Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effect of Explant Type and Different Hormonal Combinations on Direct Regeneration of Lemon balm (Melissa officinalis L.)

Document Type : Full Paper

Authors
hadijeh Jangjou 1
Abbas Hassani 1
Bahman Hosseini 1
Morad Jafari 2
Morteza Alizadeh 1
1 Department of Horticulture-Faculty of Agriculture-Urmia University
2 Department of Plant Breeding and Biothecnology-Faculty of Agriculture-Urmia University
10.22059/ijhs.2015.53489
Abstract
Lemon balm (Melissa officinalis L.) is an herbaceous perennial plant of the Lamiaceae family that has a wide edible and medicinal uses. In this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on MS mediums supplemented with different concentrations of 6-Benzylaminopurine (BAP) (2.2, 4.4 and 8.8 µm) alone or in combination with 1µm Indoleacetic acid (IAA) for direct regeneration of Lemon balm was investigated. The experiment was conducted using a factorial based on completely randomized design with three replications. The maximum mean number of shoots (23.59 and 16.90 shoots in shoot tip and nodal segment explants, respectively) was observed on the MS medium with 8.8 µm BAP. The highest regeneration rate (99%) in shoot tip and nodal segment explants was achieved on the MS medium supplemented with 8.8 and 2.2 µm BAP, respectively. No shoot regeneration occurred in other explants. The rooting of regenerated shoots was assessed on MS, ½MS and MS medium supplemented with 1, 2.5, 4.92 and 9.84 µm Indole butyric acid (IBA). The highest mean number of roots (9.06 roots per explants) was observed on the MS medium supplemented with 4.92 µm IBA.
Keywords
Lemon balm (Melissa officinalis L.)
direct regeneration
Nodal segment
BAP
MS medium
  1. Akin-Idowu, P.E., Ibitoye, D.O. & Ademoyegun, O.T. (2009). Tissue culture as a plant production technique for horticulture crops. African Journal of Biotechnology, 8(16), 3782-3788.
  2. Allahverdiyev, A., Duran, N., Ozguven, M. & Koltas, S. (2004). Antiviral activity of the volatile oils of Melissa officinalis L. against herpes simplex virus type2. Phytomedicine, 11, 657-661.
  3. Asghari, F., Hosseini. B., Hassani, A. & Shirzad, H. (2012). Effect of explants source and different hormonal combinations on direct regeneration of basil plants (Ocimum basilicum L.). Australian Journal of Agricultural Engineering, 3(1), 12-17.
  4. Ashrafuzzaman, M., Hossain, M.M., Razi Ismail, M., Shahidul Haque, M., Shahidullah, S.M. & Shahin, Z. (2009). Regeneration potential of seedling explants of chilli (Capsicum annum). African Journal of Biotechnology, 8(4), 591- 596.
  5. Ayab, M. & Sumi, S. (1998). Establishment of a novel tissue culture method, stem disk culture, and its practical application to micropropagation of garlic (Allium sativum L.). Plant Cell Reports, 17, 773-780.
  6. Baroncelli, S., Buittiet, S., Bennici, M., Foroughi, W., Mix, G., Gaul, H., Tagliasacchi, A.M., Loiero, M. & Giorgi, B. (1978). Genetic control of in vitro and in vivo growth of hexaploid wheat. Z. Pflanzenzuecht, 80, 109-116.
  7. Bennett, C. (2003). Plant extract improves cognitive function in Alzheimer's disease. Health News Co. UK.
  8. Bhojwani, S.S. & Razdan, M.K. 1996. Plant tissue culture: Theory and practice. Developments in crop science. Vol.5. Elsevier, Amsterdam.
  9. Cline, M.G. (1996). Exogenous auxin effects on lateral bud outgrowth in decapitated shoots. Annals of Botany, 78, 255-266.
  10. Dai, Y., Wang, H., Li, B., Huang, J., Liu, X., Zhou, Y., Mou, Z. & Li, J. (2006). Increased expression of mapkinase kinase causes deficiency in polar auxin transport and leads to plant architectural abnormality in Arabidopsis. Plant Cell, 18, 308-320.
  11. Debnath, M., Malik, C.P. & Bisen, P.S. (2006). Micropropagation: A tool for the production of high quality plant-based medicine. Current Pharmaceutical Biotechnology, 7, 33-49.
  12. Erdei, I., Kiss, Z. & Maliga, P. (1981). Rapid clonal multiplication of Digitalis Lanata in tissue culture. Plant Cell Reports, 1, 34- 38.
  13. Gogu, I., Diana, S.T. & Daniela, N. (2005). Investigation on the in vitro morphogenetic reaction of Melissa officinalis L. Journal of Genetic and Molecular Biology, 54, 119-122.
  14. Gulatia, A., Bharel, S., Abdin, M.Z., Jain, S.K. & Srivastava, P.S. (1996). In vitro micropropagation and flowering in Artemisia annua. Journal of Plant Biochemistry and Biotechnology, 5, 31- 37.
  15. Kabala-Dzik, A., Stojko, R., Szaflarska-Stojko, E., Wroblewska-Adamek, I., Stojko, A., Stojko, J. & Stawiarska-Pieta, B. (2004). Influence of honey balm on the rate of scare formation during experimental burn wound healing in pigs. Bulletin of the Veterinary Institute in Pulawy, 48, 311-316.
    1. Kennedy, D.O., Little, W. & Scoley, A.B. (2004). Attenuation of laboratory induced stress in humans after acute administration of Melissa officinalis L. (lemon balm). Journal of Pharmacy and Pharmacology, 56, 677-681.
    2. Larkin, P.J. & Scowcroft, W.R. (1981). Somaclonal variation a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics, 60, 197-214.
    3. Liu, C.Z., Murch, S., Jain, J.C. & Saxena, P.K. (2004). Goldenseal Hydrastis canadensis L. in vitro regeneration for germplasm conservation and elimination of heavy metal contamination. In Vitro Cellular and Developmental Biology-Plant, 70, 73–79.
  16. Meena, M., Meena, R. & Patni, V. (2010). High frequency plant regeneration from shoot tip explants of Citrullus colocynthis an important medicinal herb. African Journal of Biotechnology, 9 (31), 5037- 5041.
  17. Meftahizade, H., Lotfi, M. & Moradkhani, H. (2010a). Optimization of micropropagation and establishment of cell suspension culture in Melissa officinalis L. African Journal of Biotechnology, 9, 4314-4321.
  18. Meftahizade, H., Moradkhani, H., Naseri, B., Lotfi, M. & Naseri, A. (2010b). Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes. Journal of Medicinal Plants Research, 4, 240-246.
  19. Misra, P. & Chaturvedi, H.C. )1984(. Micropropagation of Rosmarinus officinalis L. Plant Cell Tissue and Organ Culture, 3, 163-166.
  20. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15, 473-497.
  21. Murch, S.J., Krishna, R.S. & Saxena, P.K. (2000). Tryptophan is a precursor for metalonin and serotonin biosynthesis in in vitro regenerated St. John's wort (Hypericum perforatum L. cv. Anthos) plants. Plant Cell Reports, 19, 698-704.
  22. Nagarathan, K.C., Prakash, H.S. & Shetty, H.S. (1991). Genotypic effects on the callus formation from different explants of pearl millet B lines. Advances in Plant Sciences, 4, 82-86.
  23. Nessler, C.L. (1982). Somatic embryogenesis in the Opium poppy, Papaver somniferum. Plant Physiology, 55, 453-456.
  24. Oluk, E. & Cakir, A. (2009). Micropropagation of Origanum sipyleum L. an endemic medicinal herb of Turkey. African Journal of Biotechnology, 8 (21), 5769- 5772.
  25. Omidbaigi, R. (2008). Production and processing of medicinal plants. Behnashr publisher. (In Farsi).
    1. Raoul, S., Gilbert, C., Hamidou, F.S., Yannick, T., Yao, D., Abdourahmane, S. & Michel, B. (2010). Protocols for callus and somatic embryo initiation for Hibiscus sabdariffa L. Australian Journal of Crop Science, 4, 98-106.
  26. Rech, E.L. & Pires, J.P. (1986). Tissue culture propagation of Mentha spp. by the use of the axillary buds. Plant Cell Reports, 5, 17-21.
  27. Rout, G.R., Saxena, C., Samantaray, S. & Das, P. (1999). Rapid clonal propagation of Plumbago zeylanica Linn. Plant Growth Regulation, 28, 1-4.
  28. Sarwar, S.Z., Rehman, M., Fatima, R.Z., Sial, R.A. & Chaudhary, M.F. (2009). In vitro direct regeneration in mint from different explants on half strength MS medium. African Journal of Biotechnology, 8(18), 4667- 4671.
  29. Shanshan, H., Chunzhao, L. & Praveen, S. (2007). Plant regeneration of an endangered medicinal plant Hydrastis canadensis L. Scientia Horticulturae, 113, 82-86.
  30. Tripathi, L. & Tripathi, J.N.(2003). Role of biotechnology in medicinal plant. Tropical Journal of Pharmaceutical Research, 2, 243- 253.
  31. Wake, G., Court, J., Pickering, A., Lewis, R., Wilkins, R. & Perry, E. (2000). CNS acetylcholine receptor activity in European medicinal plants traditionally used to improve failing memory. Journal of Ethnopharmacology, 69(2), 105-114.

 

 

 

 

 

 

 

Iranian Journal of Horticultural Science
Volume 45, Issue 4 - Serial Number 4
Spring 2015
Pages 391-399

  • Receive Date 28 August 2013
  • Revise Date 28 November 2013
  • Accept Date 02 December 2013