Orange varieties in Iran were accounted for the highest citrus crop production. Due to increasing of biotic and abiotic stresses, different cultivars and rootstocks should be evaluated under the conditions of region. Therefore, the quantitative and qualitative yield and harvest timing of ‘Cara Cara’ navel orange on three trifoliate rootstocks in the east of Mazandaran province were investigated during seven years. In the last year (2020), the highest morphological compatibility between scion and rootstock was observed on the ‘Carrizo’ citrange (0.76) and the lowest on the ‘Swingle’ citrumelo (0.62). Trees on ‘Swingle’ citrumelo rootstock had the highest volume (5.4 m3) and on ‘Carrizo’ citrange had the lowest (4.2 m3). The highest average yield from the fourth to seventh years was recorded on ‘Swingle’ citrumelo and the lowest on ‘C-35’ citrange (10.7 and 8.7 ton/hec, respectively). The highest yield efficiency belonged to trees on ‘Carrizo’ citrange with 4 kg/m3. The appropriate time for harvesting of this cultivar in the north of Iran is the third week of November, when the harvest index is close to 7.8, the average fruit weight is 280 g, peel thickness is 3.5 mm, fruit extract is 44%, and peel color is pale orange. According to the results, for the optimal use of the land and increasing total yield in the north of Iran, in new orchards of ‘Cara Cara’ orange on trifoliate rootstocks it is possible to use 800-1000 trees per hectare by training in the first four years and annual pruning in late winter.