اثر کاربرد پیش از برداشت بنزیل‌آدنین و نانوامولسیون فیتونسید بر صفات رویشی و زایشی گیاه و بازارپسندی میوه توت‌فرنگی (Fragaria×ananassa Duch. 'Albion')

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تخصصی علوم باغبانی و زراعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم و مهندسی خاک، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

به‌منظور بررسی تأثیر بنزیل‌آدنین و نوعی فیتونسید طبیعی بر ویژگی‌های مختلف کمی و کیفی مرتبط با بازارپسندی توت‌فرنگی، آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در گلخانه آلبیون واقع در شهرستان دماوند اجرا شد. عامل اول، بنزیل‌آدنین (6-BAP) در دو سطح (صفر و 100 میلی‌گرم در لیتر) و عامل دوم، نانوامولسیون فیتونسید (PNE) در سه سطح (صفر، 75/0 درصد و 5/1 درصد) بود. بوته‌های جوان در مرحله رشد رویشی دو نوبت با 6-BAP و در مرحلة زایشی سه نوبت با PNE، که متشکل از اسانس‌های دو گیاه کاج و  نوئل بود، محلول‌پاشی شدند. کاربرد 6-BAP در مرحلة رویشی باعث تولید تعداد برگ بیشتر و افزایش سطح برگ شد. همچنین، قطر طوقه‌ها را نسبت به گیاهان شاهد به‌طور معنی‌داری افزایش داد و بر تمام صفات مطالعه شده در مرحلة زایشی نیز اثر مثبتی داشت. این سیتوکینین، ضمن افزایش عملکرد بوته، طعم و سایر ویژگی‌های کیفی میوه را بهبود بخشید. PNE بر افزایش سفتی و ماندگاری میوه موثر بود. علاوه بر‌این، در به تعویق انداختن کاهش وزن میوه و حفظ کیفیت ظاهری آن به مدت طولانی‌تر اثر معنی‌داری داشت. بنابر نتایج، بهره-گیری از 6-BAP و فیتونسید مطالعه شده در مرحله پیش از برداشت به‌منظور بهبود ویژگی‌های میوه توت‌فرنگی توصیه می‌شود

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of Pre-harvest Application of Benzyladenine and Phytoncide Nanoemulsion on Vegetative and Reproductive Characteristics of the Strawberry (Fragaria×ananassa Duch. 'Albion') and Marketability of the Fruit

نویسندگان [English]

  • Farzad asgari 1
  • Sepideh Kalatejari 1
  • Babak Motesharezadeh 2
  • Marzieh Ghanbari Jahromi 1
  • Weria Weisany 1
1 Department of Horticultural Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Soil Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

In order to investigate the effect of benzyladenine and a kind of natural phytoncide on various quantitative and qualitative traits related to the marketing value of the strawberry (Fragaria×ananassa Duch.) plant a factorial experiment, based on a randomized complete block design was carried out with three replications in the Albion greenhouse, located in Damavand county. The first factor was benzyladenine (6-BAP) at two levels (0 and 100 mg/l) and the second, phytoncide nanoemulsion (PNE) at three levels (0, 0.75% and 1.5%). Young plants were sprayed twice with 6-BAP during the vegetative growth phase and thrice during the reproductive phase with PNE, which was comprised of the essential oils of Pinus nigra and Picea spp. Application of 6-BAP during the vegetative stage resulted in production of more leaves and higher leaf area. Also, 6-BAP increased crown diameter significantly, compared to control plants, and had a positive effect on all the studied traits at the reproductive stage, as well. This kind of cytokinin improved taste and other quality attributes of the fruits, along with plant yield. PNE was effective on increasing the fruit firmness and longevity. Furthermore, it had a significant effect on delaying the fruit weight loss and maintaining the appearance quality of the fruits for a longer time. Based on the results, taking advantage of 6-BAP and the studied PNE during pre-harvest phase is recommended for improving strawberry fruit characteristics.

کلیدواژه‌ها [English]

  • Cytokinin
  • Essential oil
  • Pre-harvest spraying

Extended Abstract

Introduction

  Strawberry (Fragaria × ananassa Duch.) is one of the most delicious and nourishing fruits of the world, with the highest production among other berries. Several factors influence the consumers' choice of strawberry fruit, including size, flavor, flesh firmness, and fruit color. Nowadays, the use of plant growth regulators is an efficient tool for improving the quantitative and qualitative properties of edible products, and if appropriate concentration is used brings great economic benefits to growers. Improving the quality of the fruit helps to increase the shelf life of the product. On the other hand, the inhibitory effect of essential oils with phytoncide properties, against plant pathogens has been proven in various plant species. Most studies to date have examined the effects of phytoncide essential oils on post-harvest period and under laboratory conditions, while pre-harvest treatment of the plant is an effective strategy in controlling post-harvest diseases of strawberries. 

Material and methods

    The current study aimed to investigate the pre-harvest application of a plant growth regulator named benzyladenine and a kind of natural phytoncide on various quantitative and qualitative traits related to the marketing value of strawberry (Fragaria×ananassa Duch.) plant. Unlike most of the previous researches, this study examines the effect of phytoncide nanoemulsion use during the crop production period. A factorial experiment, based on randomized complete block design was carried out with three replications in the Albion greenhouse, located in Damavand county. The first factor was benzyladenine (6-BAP) at two levels (0 and 100 mg/l) and the second, phytoncide nanoemulsion (PNE) at three levels (0, %0.75 and %1.5). Young plants were sprayed twice with 30 ml 6-BAP during the vegetative growth phase. The first time was 21 days after transferring the runners to grow bags, and the second 7 days later. Moreover, they were later sprayed thrice with PNE, which was comprised of the essential oils of Pinus nigra and Picea spp., during the reproductive phase at the stages of small green, big green and completely red fruits.

Results and discussion

     Application of 6-BAP during the vegetative stage, resulted in production of more leaves and higher leaf area. Also, 6-BAP increased crown diameter significantly, compared to control plants, and had a positive effect on all the studied traits at the reproductive stage, as well. This kind of cytokinin improved taste and other quality attributes of the fruits, along with plant yield. PNE was effective on increasing the fruit firmness and longevity. Furthermore, it had a significant effect on delaying the fruit weight loss and maintaining the appearance quality of the fruits for a longer time. However, there was no significant difference between the two tested concentrations of phytoncide in relation to the investigated traits.

 Conclusion

    Based on the results, taking advantage of 6-BAP and the studied PNE during pre-harvest phase is recommended for improving various characteristics of strawberry fruit and extending its post-harvest life.

Abdi, S., Abbaspur, N., Avestan, S., & Barker, A. V. (2016). Sana physiological responses of two grapevine (Vitis vinifera L.) cultivars to CycocelTM treatment during drought. The Journal of Horticultural Science and Biotechnology, 91(3), 211-219.
Ali, M. M., Anwar, R., Malik, A. U., Khan, A. S., Ahmad, S., Hussain, Z., Ul Hasan, M., Nasir, M. & Chen, F. (2021). Plant growth and fruit quality response of strawberry is improved after exogenous application of 24-epibrassinolide. Journal of Plant Growth Regulation, 1-14.
Aremu, A. O., Fawole, O. A., Makunga, N. P., Masondo, N. A., Moyo, M., Buthelezi, N. M., Amoo, S.O., Spíchal, L. & Doležal, K. (2020). Applications of cytokinins in horticultural fruit crops: Trends and future prospects. Biomolecules, 10(9), 1222.
Asghari Marjanlu, A., Mostofi, Y., Shoeibi, S. H. & Maghoumi, M. (2009). Effect of basil (Ocimum basilicum L.) essential oil on gray mold control and postharvest quality of strawberry (cv. Selva). Journal of Medicinal Plants, 8(28), 131-139. (In Persian)
Ather-uz-Zaman, Al-Khayri J.M. & Islam R. (2018). Genetic improvement of strawberry (Fragaria × ananassa Duchesne). In: Al-Khayri J., Jain S., Johnson D. (eds) Advances in Plant Breeding Strategies: Fruits. Springer, Cham.
Azadshahraki, F. & Kaffashan, J. (2017). Qualitative indices of horticultural crops and their measurement methods (1st Ed.). Agricultural research, Education and extension organization, Karaj. (In Persian)
Bal, E., & Ürün, B. A. (2021). Effects of chitosan coating with putrescine on bioactive compounds and quality of strawberry cv. San Andreas during cold storage. Erwerbs-Obstbau, 63(1), 7-14.
Canli, F. A., & Pektas, M. (2015). Improving fruit size and quality of low yielding and small fruited pear cultivars with benzyladenine and gibberellin applications. European Journal of Horticultural Science, 80(3), 103-108.
Capozzi, F., Sorrentino, M. C., Di Palma, A., Mele, F., Arena, C., Adamo, P., Spagnuolo, V. & Giordano, S. (2020). Implication of vitality, seasonality and specific leaf area on PAH uptake in moss and lichen transplanted in bags. Ecological Indicators, 108, 105727.
Chandler, C. K., Folta, K., Dale, A., Whitaker, V. M., & Herrington, M. (2012). Strawberry. In: Badenes, M., Byrne, D. (Eds), Fruit breeding. 305-325. Springer, Boston, MA.
Cheng, W. W., Lin, C. T., Chu, F. H., Chang, S. T., & Wang, S. Y. (2009). Neuropharmacological activities of phytoncide released from Cryptomeria japonica. Journal of Wood Science, 55(1), 27-31.
Cruz-Rus, E., Amaya, I., Sanchez-Sevilla, J. F., Botella, M. A., & Valpuesta, V. (2011). Regulation of L-ascorbic acid content in strawberry fruits. Journal of Experimental Botany, 62(12), 4191-4201.
Gan, L., Song, M., Wang, X., Yang, N., Li, H., Liu, X., & Li, Y. (2022). Cytokinins are involved in regulation of tomato pericarp thickness and fruit size. Horticulture research, 9.
Garrido-Bigotes, A., Figueroa, P. M., & Figueroa, C. R. (2018). Jasmonate metabolism and its relationship with abscisic acid during strawberry fruit development and ripening. Journal of plant growth regulation, 37(1), 101-113.
Hancock J., Sjulin T., Lobos G. (2008) Strawberries. In: Hancock J.F. (Ed) Temperate fruit crop breeding. Springer, Dordrecht.
Hönig, M., Plíhalová, L., Husičková, A., & Doležal, K. (2018). Role of cytokinins in senescence, antioxidant defense and photosynthesis. International journal of molecular sciences, 19(12), 4045.
Hosseini, S., Amini, J., Saba, M. K., Karimi, K., & Pertot, I. (2020). Preharvest and postharvest application of garlic and rosemary essential oils for controlling anthracnose and quality assessment of strawberry fruit during cold storage. Frontiers in Microbiology, 11, 1855.
Kasem, M. M., & Helaly, A. A. E. (2021). Response of Syngonium podophyllum plant to some synthetic
 cytokinin types and concentrations as a foliar application. Scientific Journal of Flowers and Ornamental Plants, 8(3), 321-334.
Kim, D. H., Kim, H. B., Chung, H. S., & Moon, K. D. (2014). Browning control of fresh-cut lettuce by phytoncide treatment. Food Chemistry, 159, 188-192.
Kour, S., Kumar, R., Wali, V., Sharma, A., & Bakshi, P. (2017). Impact of benzyladenine and gibberellic acid on quality and economics of runner production in Chandler strawberry (Fragaria× ananassa) under subtropical climate. Indian Journal of Agricultural Sciences, 87, 964-967.
Kurkina, Y., Esina, E., & Barskova, A. (2020). Phytoncidal activity of essential oils of medicinal plants to some strains of mold fungi. Journal of Agriculture and Environment, 1(13):21-24.
Lester, G. E., Lewers, K. S., Medina, M. B., & Saftner, R. A. (2012). Comparative analysis of strawberry total phenolics via Fast Blue BB vs. Folin–Ciocalteu: Assay interference by ascorbic acid. Journal of Food Composition and Analysis, 27(1), 102-107.
Li, Y., Hu, J., Xiao, J., Guo, G., & Jeong, B. R. (2021). Foliar thidiazuron promotes the growth of axillary buds in strawberry. Agronomy, 11(3), 594.
Li, Y., Hu, J., Wei, H., & Jeong, B. R. (2020). A long-day photoperiod and 6-benzyladenine promote runner formation through upregulation of soluble sugar content in strawberry. International journal of molecular sciences, 21(14), 4917.
Li, L., Li, D., Luo, Z., Huang, X., & Li, X. (2016). Proteomic response and quality maintenance in postharvest fruit of strawberry (Fragaria× ananassa) to exogenous cytokinin. Scientific reports, 6(1),1-11.
Liu, C., Guo, Z., Park, Y. G., Wei, H., & Jeong, B. R. (2019). PGR and its application method affect number and length of runners produced in ‘Maehyang’ and ‘Sulhyang’ strawberries. Agronomy, 9(2), 59.
Liu, C., Zheng, H., Sheng, K., Liu, W., & Zheng, L. (2018). Effects of melatonin treatment on the postharvest quality of strawberry fruit. Postharvest Biology and Technology, 139, 47-55.
Mahajan, B.V.C., Tandon, R., Swati, K. & Sidhu, M.K. (2018). Natural coatings for shelf-life enhancement and quality maintenance of fresh fruits and vegetables - A review. Journal of Postharvest Technology, 6(1): 12–26.
Martínez, K., Ortiz, M., Albis, A., Gilma Gutiérrez Castañeda, C., Valencia, M. E., & Grande Tovar, C. D. (2018). The effect of edible chitosan coatings incorporated with Thymus capitatus essential oil on the shelf-life of strawberry (Fragaria x ananassa) during cold storage. Biomolecules, 8(4), 155.
Medan, R. A., & Al-Douri, E. F. S. (2021). Effect of foliar application of Sitofex, potassium and arginine on vegetative growth of “Zaghinia” apricot trees. In IOP conference series: Earth and Environmental Science, 761(1):  p. 012053. IOP Publishing.
Mostafa, S. H., & Brengi, A. (2018). Growth, yield and chemical composition of okra as affected by Three types and levels of synthetic cytokinins under high temperature conditions. Alexandria Journal of Agricultural Sciences, 63(6), 365-372.
Nguyen, V. T., Nguyen, D. H., & Nguyen, H. V. (2020). Combination effects of calcium chloride and nano-chitosan on the postharvest quality of strawberry (Fragaria x ananassa Duch.). Postharvest Biology and Technology, 162, 111103.
Ni, J., Shah, F. A., Liu, W., Wang, Q., Wang, D., Zhao, W., Lu, W., Huang, S., Fu, S. & Wu, L. (2018). Comparative transcriptome analysis reveals the regulatory networks of cytokinin in promoting the floral feminization in the oil plant Sapium sebiferumBMC plant biology, 18(1), 1-16.
Palta, J. P. (1990). Leaf chlorophyll content. Remote sensing reviews, 5(1), 207-213
Pandey, S., Giri, V. P., Tripathi, A., Kumari, M., Narayan, S., Bhattacharya, A., Srivastava, S.  & Mishra, A. (2020). Early blight disease management by herbal nanoemulsion in Solanum lycopersicum with bio-protective manner. Industrial Crops and Products, 150, 112421.
Pétriacq, P., López, A., & Luna, E. (2018). Fruit decay to diseases: can induced resistance and priming help?. Plants, 7(4), 77.
Ragini, B. K., Chandrashekar, S. Y., Hemla, N. B., Shivaprasad, M., & Ganapathi, M. (2019). Effect of cytokinins (benzyl adenine and kinetin) on bulbous flower crops: A review. International Journal of Chemical Studies, 7(5), 2618-2622.
Robles-Flores, G. D. C., Abud-Archila, M., Ventura-Canseco, L. M. C., Meza-Gordillo, R., Grajales-Lagunes, A., Ruiz-Cabrera, M. A., & Gutiérrez-Miceli, F. A. (2018). Development and evaluation of a film and edible coating obtained from the Cajanus cajan seed applied to fresh strawberry fruit. Food and Bioprocess Technology, 11(12), 2172-2181.
Rojas, B., Suárez-Vega, F., Saez-Aguayo, S., Olmedo, P., Zepeda, B., Delgado-Rioseco, J., Defilippi, B.G., Pedreschi, R., Meneses, C., Pérez-Donoso, A.G.  & Campos-Vargas, R. (2021). Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides. Plants, 10(12), 2642.
Savini, G., Neri, D., Zucconi, F., & Sugiyama, N. (2005). Strawberry growth and flowering: an architectural model. International Journal of Fruit Science, 5(1), 29-50.
Seifi, F., Farzaneh, M., Rafati, H. & Rezadoost, H. (2014). Antifungal potency of some medicinal plants essential oils nanoemulsions to control soft rot in strawberry fruit caused by Rhizopus stolonifer. Biocontrol in Plant Protection, 2(1), 69-79. (In Persian)
Shahavi, M. H., Hosseini, M., Jahanshahi, M., Meyer, R. L., & Darzi, G. N. (2016). Clove oil nanoemulsion as an effective antibacterial agent: Taguchi optimization method. Desalination and Water Treatment, 57(39), 18379-18390.
Shams, M., Yildirim, E., Ekinci, M., Agar, G., Turan, M., & Kul, R. (2018). Exogenous cytokinin application increased the capsaicin and ascorbic acid content in pepper fruit. Sci Papers, 62, 507-11.
Shaw, D.V. & Larson, K.D. (2006). Strawberry plant named ʻAlbionʼ. U.S. Patent No. US PP16,228 P3, 31 January 2006.
Souri, M. K., & Bakhtiarizade, M. (2019). Biostimulation effects of rosemary essential oil on growth and nutrient uptake of tomato seedlings. Scientia Horticulturae, 243, 472-476.
Sun, Y., Asghari, M., & Zahedipour-Sheshgelani, P. (2020). Foliar spray with 24-epibrassinolide enhanced strawberry fruit quality, phytochemical content, and postharvest life. Journal of Plant Growth Regulation, 1-10.
Taheri, M., & Haghighi, M. (2018). Benzyl adenine is more effective than potassium silicate on decreasing the detrimental effects of heat stress in pepper (Capsicum annum cv. PS301). Iran Agricultural Research, 37(1), 89-98.
Tatiana, P., Odagiu, A., Mălinaş, C., & Balint, C. (2020). The potential phytoncide effect of Allium cepa L. extracts. ProEnvironment Promediu, 13(44),133-136.
Wei, Y., Shao, X., Wei, Y., Xu, F., & Wang, H. (2018). Effect of preharvest application of tea tree oil on strawberry fruit quality parameters and possible disease resistance mechanisms. Scientia Horticulturae, 241, 18-28.
Wu, W., Du, K., Kang, X., & Wei, H. (2021). The diverse roles of cytokinins in regulating leaf development. Horticulture Research, 8.
Zahedipour-Sheshglani, P., & Asghari, M. (2020). Impact of foliar spray with 24-epibrassinolide on yield, quality, ripening physiology and productivity of the strawberry. Scientia Horticulturae, 268, 109376.
Zhang, Y., Liu, Y., Hu, W., Sun, B., Chen, Q., & Tang, H. (2018). Anthocyanin accumulation and related gene expression affected by low temperature during strawberry coloration. Acta Physiologiae Plantarum, 40(11), 1-8.