اثر هرم‌سازی ژن‌های مقاومت به ویروس پیچیدگی برگ زرد گوجه‌فرنگی (‏Tomato yellow leaf ‎curl virus, TYLCV‏) بر رنگدانه‌های فتوسنتزی و برخی صفات فیزیولوژی در لاین‌های مختلف‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه هرمزگان، بندرعباس، ایران ‏

2 استاد، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران

چکیده

بیماری ویروسی پیچیدگی برگ زرد گوجه­فرنگی (TYLCV) یکی از خسارت­زاترین عواملی است که در سرتاسر جهان باعث تلفات بسیار بالایی به محصول گوجه­فرنگی می­شود و در بیشتر مناطق ایران نیز پراکنش یافته است. راهکارهای متعددی برای مقابله با این بیماری صورت گرفته که عملاً این روش­ها ناکارآمد بوده­اند. به منظور بررسی نقش انتقال و ترکیب دو مکان ژنی TY-2 و TY-1/3 در تعدادی لاین حساس و مطلوب مطالعه حاضر صورت پذیرفت. نتایج نشان داد لاین­های حاوی دو مکان ژنی TY-2 و TY-1/3 میزان خسارت کمتری را در میزان کلروفیل و محتویات پروتئین کل خود تجربه کردند. در بررسی میزان پرولین و میزان بیان ژن PAL مشاهده شد لاین­های حاوی دو مکان ژنی سطح کمتری از میزان پرولین و بیان ژن PAL را در خود نشان دادند. همچنین ردیابی آلودگی ویروسی به وسیله واکنش زنجیره پلیمراز (PCR) با استفاده از آغازگر­های اختصاصی بیانگر عدم تکثیر ژنوم ویروس در لاین­های حاوی هر دو مکان ژنی بود. در مجموع نتایج این تحقیق بیانگر نقش بالای حضور و هرم سازی دو مکان ژنی TY-2 و TY-1/3 به منظور ایجاد مقاومت در گوجه­فرنگی در برابر TYLCV می­باشد که منجر به کاهش خسارت در برابر این ویروس می­شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of pyramiding resistance control genes on viral disease of tomato yellow ‎leaf curl virus (TYLCV) on photosynthetic pigments and some physiological traits in ‎different lines

نویسندگان [English]

  • Kamal Salehi 1
  • Gholam-Reza Sharifi-Sirchi 2
1 Ph.D. Candidate, College of Agriculture, University of Hormozgan, Bandar Abbas, Iran‎
2 Professor, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran‎
چکیده [English]

Tomato yellow leaf viral disease (TYLCV) is one of the most damaging agents that causes very high yield losses worldwide and has spread to most parts of Iran. Numerous strategies have been developed to combat this disease, but these methods have been practically ineffective. In order to investigate the role of transmission and combination of TY-2 and TY-1/3 gene loci in a number of sensitive and desirable line, the present study was performed.Results showed that the lines containing the two TY-2 and TY-1/3 gene loci experienced less damage regarding chlorophyll content and total protein content. In the study of proline content and PAL gene expression, it was observed that lines containing two gene loci showed lower levels of proline and gene expression. Also, in order to detect viral infection by polymerase chain reaction (PCR) using specific primers, it indicated that the virus genome does not replicate in the lines containing both gene loci. In general, the results of this study indicate the high role of the presence and pyramiding of two gene sites TY-2 and TY-1/3 in order to create resistance in tomato against TYLCV and lead to a reduction in damage against this virus.

کلیدواژه‌ها [English]

  • Gene pyramiding
  • gene locations Ty-2 and TY-1/3
  • PAL gene
  • photosynthetic pigments
  • resistance‎

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علوم باغبانی ایران
دوره 53، شماره 4
دی 1401
صفحه 837-848

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سابقه مقاله

  • تاریخ دریافت: 02 آذر 1400
  • تاریخ بازنگری: 25 دی 1400
  • تاریخ پذیرش: 05 بهمن 1400

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