بررسی کنترل ژنتیکی عادت رشد در نتاج حاصل از تلاقی کدو خورشتی و کدو پوست تخم کاغذی با ‏استفاده از تجزیه میانگین نسل‌ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده علوم کشاورزی، دانشگاه گیلان، گیلان، ایران ‏

2 دانشیار، دانشکده علوم کشاورزی، دانشگاه گیلان، گیلان، ایران ‏

3 استاد، دانشکده علوم کشاورزی، دانشگاه گیلان، گیلان، ایران ‏

4 دانشیار، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران ‏

چکیده

 انتخاب روش به نژادی مناسب به نوع و مقدار نسبی اجزای ژنتیکی بستگی دارد. در این مطالعه شش نسل P1، P2، F1، F2،BC1  و BC2 از تلاقی دو لاین کدو پوست تخم کاغذی و کدو خورشتی (S10×P25) تهیه و از نظر صفت طول ساقه ارزیابی شدند. هم­زمان صفات مربوط به عملکرد و اجزای آن (تعداد میوه در هکتار، تعداد بذر در میوه، وزن میوه و وزن صددانه) نیز ارزیابی شد. آزمایش در قالب طرح بلوک­های کامل تصادفی با سه تکرار در سال 1398 انجام شد. نتایج نشان داد تفاوت معنی­داری بین نسل­ها از نظر طول ساقه وجود داشت. آزمون­های مقیاس و مقیاس مشترک حاکی از وجود اپیستازی برای این صفت بود. واریانس افزایشی در رابطه با اکثر صفات از اهمیت بیشتری برخوردار بود، به این معنی که احتمالا گزینش می­تواند روش مناسبی برای بهبود این صفات باشد. در هیبرید F1 از نظر همه صفات هتروزیس بالا مشاهده شد. وراثت‌پذیری خصوصی برای همه صفات بالا به­دست آمد که نشان از اهمیت انتخاب در بهبود صفات بود. بنابراین با توجه به نتایج حاصل از آنالیز میانگین نسل‌ها و برآورد آثار ژنتیکی صفات به ویژه صفت مربوط به عادت رشدی و رسیدن به طول ساقه کوتاه­تر می­توان روش انتخاب را به کار گرفت.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the genes actions on growth habits of pumpkin in crossing of zucchini ‎and hull-less seeded pumpkin using generation mean analysis

نویسندگان [English]

  • Sima Davoodi 1
  • Jamal-Ali Olfati 2
  • Babak Rabiei 3
  • Atefeh Sabouri 2
  • Ghaffar Kiani 4
1 Ph. D. Student,, Faculty of Agricultural Science, University of Guilan, Guilan, Iran
2 Associat Professor, Faculty of Agricultural Science, University of Guilan, Guilan, Iran
3 Professor, Faculty of Agricultural Science, University of Guilan, Guilan, Iran
4 Associate Professor, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
چکیده [English]

Selecting an appropriate breeding method depends on the type and relative proportion of genetic components. In this study, six generations P1, P2, F1, F2, BC1 and BC2 from a cross between two cucurbita varieties crossing of hull-less seed pumpkin and squash, S10×P25, were constructed and evaluated for main stem length. Yield and its components (number of fruit per hectare, number of seeds per fruit, fruit weight and 100- seed weight) were also evaluated. The experiment was carried out in a randomized complete blocks design with three replications in 2019. The results showed significant differences among generations for main stem length. Scaling and Chi-square (χ2) tests suggests the presence of digenic epistasis for this trait. According to the results, additive variance was more important for most of the traits, meaning that selection is a good way to improve these traits. High heterosis was observed in the F1 hybrid for all of the traits. High narrow-sense heritability estimates were shown for all of the traits. Therefore, according to the results of estimating the genetic effects of traits, especially traits related to growth habit and reaching a shorter stem length, the selection method can be used.

کلیدواژه‌ها [English]

  • Additive effect
  • dominant effect
  • epistasis
  • narrow-sense heritability
  • selection

مراجع

  1. Al-Araby, A. A. (2004). Breeding studies on cucumber crop (Cucumis sativus ). M.Sc. Thesis, Faculty of Agriculture, Tanta. University.
  2. Al-Ballat, I. A. (2008). Breeding studies on summer squash crop (Cucurbita pepo ). M.Sc. Thesis, Faculty of Agriculture, Tanta. University.
  3. Amaefula, C., Agboand, C. U. & Nwofia, G. E. (2014). Hybrid vigour and genetic control of some quantitative traits of tomato (Solanum lycopersicum ). Open Journal of Genetics, 4, 30-39.
  4. Anant, S.O. (2018). Generation mean analysis in Bitter gourd (Momordica charantia). M.Sc. Thesis, College of Agriculture, Latur, India.
  5. Aruah, C., Uguru, M. & Oyiga, B. (2010). Variations among some Nigerian Cucurbita African Journal of Plant Science, 4, 374-386.
  6. Azizi, F., Rezaie, A. & Saeidi, G. (2006). Generation mean analysis to estimate genetic parameters for different traits in two crosses of corn inbred lines at three planting densities. Journal of Agricultural Science and Technology, 8, 153-169.
  7. Bahlgerdi M., Aroiee H. & Azizi M. (2014). The study of plant density and planting methods on some growth characteristics, seed and oil yield of medicinal pumpkin (Cucurbita pepo styriaca, cv. ‘Kaki). American Journal of Life Sciences, 2(5), 319-324.
  8. Bernardo, R. (2002). Breeding for Quantitative Traits in Plants. Stemma Press.
  9. Buhler, D. D. (2009). 50th Anniversary-Invited Article: Challenges and opportunities for integrated weed management. Weed Science, 50 (3), 273-280.
  10. Cushman, K. E., Horgan, T. E., Nagel, D. H. & Gerard, P. D. (2004). Plant population affects pumpkin yield components. Horticultural Technology, 1, 326-331.
  11. Darrudi, R., Nazeri, V., Soltani, F., Shokrpour, M., & Ercolano, M.R. (2018). Evaluation of combining ability in Cucurbita pepo and Cucurbita moschata Duch. accessions for fruit and seed quantitative traits. Journal of Applied  Research on. Medicinal and Aromatic Plants, 9, 70-77.
  12. Davoodi, S., Olfati, J.A., HamidOghli, Y. & Sabouri, A. (2015). Evaluation of yield and quality of some hybrids between Squash (Cucurbita moschata) and Pumpkin (Cucurbita pepo L.). M.Sc. Thesis. Faculty of Agricultural Sciences. University of Guilan. Rasht. Iran.
  13. Doane, T.A., Horwath W.R., Mitchell J.P., Jackson J., Miyao G. & Brittan, K. )2009(. Nitrogen supply from fertilizer and legume cover crop in the transition to no-tillage for irrigated row crops. Nutrient Cyclingin Agroecosystems, 85, 253-262.
  14. Elias, M. S., Hassan, K. D., Odeh, S. & Mohiaddin, S. R. (2020). Study of growth, yield and phytosterol of Squash (Cucurbita pepo) and medical Pumpkin (Cucurbita pepo L.) and their hybrid. Iraqi Journal of Agricultural Sciences, 51(2),675-684
  15. El-Hamed, K. E. S. A. & Elwan, M. W. M. (2011). Dependence of pumpkin yield on plant density and variety. American Journal of Plant Sciences, 2(5), 636-643.
  16. Fereshtian, M., Salehi, R., Kashi & Babalar M. (2020). Preliminary evaluation of compatibility, growth and yield of Khatooni melon’s scion on different cucurbit rootstocks in the field condition. Iranian Journal of Horticultural Science, 52(2), 447-459. .(In Farsi).
  17. Gonçalves, L. S., Rodrigues, A. R., dos Santos Bento, C., Robaina, R.R. & do Amaral Júnior, A. T., (2011). Herança de caracteres relacionados à produção de frutos em Capsicum baccatum pendulum com base em análise dialélica de Hayman. Revista Ciência Agronômica, 42(3), 662-669.
  18. Ghorbani, R., Khorramdel S., Asadi G.A & African R. (2017). Effect of non-chemical weed management strategies on population and diversity index for weeds in spinach. Journal of Agroecology, 9(3), 638-651. (In Farsi).
  19. Hasandokht, R. (2012). Vegetable Production Technology (1th ed.). Selseleh press.
  20. Hayman, B. I. (1958). The separation of epistasis from additive and dominance variation in generation Means. Heredity, 12(3), 371-390.
  21. Hosseinzadeh Colagar, A. & Amjadi Souraki, O. (2012). Review of Pumpkin anticancer effects. Quran  and Medicine, 1(4), 93-104.
  22. Javanmard, T., Soltani Saleh-abadi, F. & Bihamta, M. R. (2018). Estimation of some genetic parameters through generation mean analysis in Melon. Indian Journal of Agricultural Research, 52,619-624
  23. Jinks, J.H. & Jones, R.M. (1958). Estimation of components of heterosis. Genetics, 43(2), 223-234.
  24. Kang, M.S. (2003). Handbook of formulas and software for plant geneticists and tree breeders. Food Products Press.
  25. Kaur, N. (2016). Genetic analysis of important economictraits and validation of molecular markers linked to Hull-less Seed traits in Pumpkin (Cucurbita pepo pepo var. styriaca). Ms.C. Thesis. Punjab Agricultural University, Ludhiana.
  26. Kearsey, M. J. & Pooni, H. S. (1996). The genetical analysis of quantitative traits. Chapman and Hull, London. UK. pp.380.
  27. Kuabara, M.Y. (1984). Interspecific hybrids of cucurbita obtained by embryo culture. Escola Superior de Agriculture Luis Queiroz. Brazil. pp.69.
  28. Kumar, R. & Wehner, T. C. (2013). Quantitative analysis of generations for inheritance of fruit yield in watermelon. Horticultural Science, 48(7), 844-847.
  29. Latifi, M., Barimavandi, A., Sedaghathoor, S. & Lipayi, S.R. (2012). Sowing date and plant population effects on seed yield of Cucurbita pepo. International Journal of Agriculture and Biology, 14, 641-644.
  30. Lόpez Anido, F., Cravero, V., Asperelli, P., Firpo, T., Garcia, S. M. & Cointry, E. (2004). Heterotic patterns in hybrids involving cultivar-groups of summer-squash, Cucurbita pepo Euphytica 135(3), 355-360.
  31. Loy, J.B. (2004). Morpho-physiological aspects of productivity and quality in squash and pumpkins (Cucurbita). Critical reviews in plant sciences, 23(4):337-363.
  32. Madadkhah, E., Bolandnazar, S. & Oustan, S. (2017). Effect of salt stress on growth, antioxidant enzymes activity, lipid peroxidation and photosystem II efficiency in cucumber grafted on cucurbit rootstock. Iranian Journal of Horticultural Science, 49(2), 465-475. .(In Farsi).
  33. Mather, K. & Jinks, J. L. (1982). Biometrical genetics (3th ed.). Champan and Hall.
  34. Meybodi, S.A.M. (2007). Principles and basics of plant breeding (1th ed.). Isfahan University of Technology Publications.
  35. Navjot, K. (2018). Genetic studies of yield and its component traits using generation mean analysis in summer squash (Cucurbita pepo pepo).Vegetable Science, 45(2), 154-160
  36. Nesom, G.L. (2011). New state records for Citrullus, Cucumis, and Cucurbita (Cucurbitaceae) outside of cultivation in the USA. Phytoneuron, 1, 1-7.
  37. Obiadalla-Ali, H. A. (2006). Heterosis and nature of gene action for earliness and yield components in summer squash (Cucurbita pepo ). Assiut Journal of Agricultural Science, 37, 123-135.
  38. Paris, H.S. (2002). Multiple allelism at a major locus affecting fruit coloration in Cucurbita pepo. Euphytica, 125, 149-153.
  39. Paris, H.S. & Kabelka, E. (2009). Gene list for Cucurbit a Cucurbit Genetics Cooperative Report, 31-32, 44-69.
  40. Paris, H., Yonah, S., Portony, N., Mozes-Daube, V., Tzuri, N. & Katzir, G. (2003). Assessment of genetic relationships in (Cucurbita pepo) Cucurbitaceae using DNA markers. Theoretical and Applied Genetics, 106, 971-78.
  41. Pandey, J. B. & Rao, T.V.R. (2010). Analysis of certain biochemical changes associated with growth and ripening of pumpkin fruit in relation to its seed development. Journal of Pure and Applied Science, 18, 34-39.
  42. Peyvast, G.A. (2006). Olericulture (5th ed.). Daneshpazar press.
  43. Ranjbar, G.A. (2012). Theoretical and Practical Plant Breeding (2nd ed). Mazandaran University Press.
  44. Rassam, G., Naddaf, M. & Sefidcon, F. (2007). Effect of planting date and plant density on yield and seed yield components of anise (Pimpinella anisum ). Pajouhesh & Sazandegi, 75, 127-133.(In Farsi).
  45. Rebolloza-Hernández, H., Castillo-Gutiérrez, A. & Carapia-Ruíz, V.E. (2016). Estimation of genetic parameters and selection of S1 lines in a segregating population of tropical maize. Revista Mexicana de Ciencias Agrícolas, 7(8), 1893-1904.
  46. Saad, M. S. (2003). Inheritance of some economical traits in Squash (Cucurbita pepo ). Ms.C. Thesis, Faculty of Agricultural Mansoura University, Egypt.
  47. Savage, J.A., Haines, D.F. & Holbrook, N.M. (2015). The making of giant pumpkins: how selective breeding changed the phloem of Cucurbita maxima from source to sink. Plant, Cell & Environment, 38 (8), 1543-1554.
  48. Swanton, C. J. & Weise, S. F. (1991). Integrated weed management: the rationale and approach. Weed Technology, 5(3), 657-663.
  49. (2016). Descriptors for Cucurbits guidelines for the conduct of tests for distictness, uniformity and stability Pumpkin (Cucurbita pepo L.). International union for the protection of new varieties of plants, 29 pp.
  50. Villanueva-Verduzco, C., Ayala-Esteban, J. A., Villanueva-Sánchez, E., Sahagen-Castellanos, J., Sánchez Cabrera, I., Chaning Merrick, L. & Guadalupe Irizar Garzas, M. B. (2020). Changes of genetic variances and heritability by effect of selection in a Mexican local variety of Squash. Journal of Agriculture and Horticulture Research, 7(5), 225-230.
  51. Walker, R. H. & Buchanan, G. A. (1982). Crop manipulation in integrated weed management systems. Weed Science, 30(1), 17-24.
  52. Wu, T., Zhou, J., Zhang, Y. & Cao, J. (2007). Characterization and inheritance of a bush-type in tropical pumpkin (Cucurbita moschata ). Scientia Horticulture, 114, 1-4.
  53. Zraidi, A., Pachner, M., Lelley, T. & Obermayer, R. (2003). On the genetics and histology of the Hull-less character of styrian Oil-pumpkin (Cucurbita pepo). Cucurbit Genetics Cooperative Report, 26, 57-61.
  54. Zraidi, A., Stift, G., Pachner, M., Shojaeiyan, A., Gong, L. & Lelley, T. (2007). A consensus map for Cucurbita pepo Molecular Breeding, 20(4), 375-388.
علوم باغبانی ایران
دوره 53، شماره 2
شهریور 1401
صفحه 453-464

فایل ها

سابقه مقاله

  • تاریخ دریافت: 04 دی 1399
  • تاریخ بازنگری: 09 مهر 1400
  • تاریخ پذیرش: 09 آبان 1400

هم رسانی

ارجاع به این مقاله

آمار