اثر اسیدآبسیزیک بر عملکرد، فعالیت آنزیم‌های آنتی‌اکسیدانی و محتوای پرولین گیاه اسطوخودوس‎ ‎‏(‏Lavendula angustifolia cv. Organic Munestead‏) در واکنش به ‌کم‌آبیاری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 استادیار، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 دانشیار، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

4 استادیار، مرکز ملی مهندسی ژنتیک و بیوتکنولوژی، تهران، ایران

چکیده

خشکی از مهمترین عوامل محدودکننده رشد گیاهان در سرتاسر جهان و شایع‌ترین تنش محیطی بوده و اسیدآبسیزیک (ABA) نقش مهمی در راه‌اندازی و هماهنگی بسیاری از فرآیندهای فیزیولوژیکی و بیوشیمیایی در کاهش تنش اکسیداتیو دارد. به‌منظور مطالعه اثر محلول‌پاشی اسیدآبسیزیک بر عملکرد، فعالیت آنزیم‌های آنتی‌اکسیدانی و محتوای پرولین اسطوخودوس (Lavendula angustifolia cv. Organic Munestead) تحت تنش کم‌آبیاری، آزمایشی گلدانی به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار به‌اجرا درآمد. تیمارهای آزمایش در چهار سطح رژیم‌های آبیاری (شامل 100-90، 80-70، 60-50 و 40-30 درصد ظرفیت ‌زراعی (FC)) و سه سطح محلول‌پاشی ABA (شامل صفر، 15 و 30 میکرومولار در لیتر) لحاظ گردید. صفات در مرحله گلدهی اندازه‌گیری شدند. کم‌آبیاری در بیشترین سطح، منجربه افزایش91/0درصدی پرولین و کاهش 88/29 درصدی کارتنوئید برگ نسبت به شاهد گردید. ABA نیز بر تمامی صفات موردبررسی، بجز کارتنوئید، اثر معنی‌دار داشت. بیشترین فعالیت CAT، POD و SOD در 30 میکرومولار در لیتر ABA در شرایط رژیم آبیاری 40-30 درصد FC حاصل شد، درحالی‌که بیشترین میزان فعالیت آنتی‌اکسیدانی‌کل (54/70 میلی‌‌گرم بر گرم وزن خشک) از کاربرد 15 میکرومولار در لیتر ABA در 60-50 درصد FC و پرولین (51/2 میلی‌گرم بر گرم وزن خشک) از محلول‌پاشی 15 میکرومولار ABA در 40-30 درصد FC مشاهده شد. بیشترین میزان وزن تازه و خشک اندام هوایی و وزن خشک ریشه از عدم کاربرد  ABAدر 100-90 درصد FC حاصل شد. می‌توان بیان داشت که ABA در شرایط تنش کم آبیاری با افرایش فعالیت آنزیم های آنتی اکسیدانی، باعث کاهش اثرات منفی تنش کم آبیاری در اسطوخودوس شد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Abscisic acid regulator on yield, antioxidant enzymes activity and ‎proline content of lavender (Lavendula angustifolia cv. Organic Munestead) in ‎response to deficit irrigation

نویسندگان [English]

  • Hossein Gorgini Shabankareh 1
  • Sarah Khorasaninejad 2
  • Hassan Soltanloo 3
  • Vahid Shariati 4
1 Ph. D. Candidate, Gorgan University of Agricultural Sciences and Natural Resources, ‎Gorgan, Iran
2 Assistant Professor, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Associate Professor, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Assistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
چکیده [English]

Drought is one of the most important factors limiting plant growth worldwide and the most common environmental stress. In response to environmental stresses, abscisic acid (ABA) plays an important role in initiating and coordinating many physiological and biochemical processes in reducing oxidative stress. To study the effects of abscisic acid on some antioxidant enzymes and proline of Lavendula angustifolia cv. Organic Munestead under deficit irrigation stress, a pot experiment was conducted as factorial based on randomized complete block design with three replications. The treatments were four levels of irrigation regimes (30-40, 50-60, 70-80 and 90-100 percent field capacity (FC)) and ABA including three levels (0, 15 and 30 mM/L). Traits evaluated in flowering stage. The highest level of drought resulted an increasing 91% in proline and decreasing 29.88% in carotenoid compared to control. Abscisic acid had a significant effect on all traits, except of carotenoid content. Abscisic acid 30 mM/L spray and irrigation regimes 30-40 percent FC induced the highest content of CAT, POD and SOD, while the maximum of antioxidant activity (70.54 mg/g DW) has resulted in ABA 15 Mm/L application and irrigation regimes 50-60 percent FC and the maximum of proline (2.51 mg/g DW) has resulted in ABA 15 mM/L application and irrigation regimes 30-40 percent FC. The highest fresh and dry weight of shoot and root were obtained from non-application of ABS in irrigation of 90-100% FC. It is determined abscisic acid application in deficit irrigation stress conditions increased the activity of antioxidant enzymes of lavender to counteract the negative effects of deficit irrigation stress.

کلیدواژه‌ها [English]

  • Antioxidant
  • anthocyanin
  • ascorbate peroxidase
  • catalase
  • peroxidase
  • superoxide dismutase‎

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علوم باغبانی ایران
دوره 52، شماره 1
خرداد 1400
صفحه 195-211

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سابقه مقاله

  • تاریخ دریافت: 20 آبان 1398
  • تاریخ بازنگری: 10 دی 1398
  • تاریخ پذیرش: 05 بهمن 1398

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