بررسی تغییرات میزان فعالیت آنتی‌اکسیدانی، ترکیبات فنلی، رنگیزه‌های فتوسنتزی و صفات کمی میوه ‏در چهار ژنوتیپ‌ وحشی تمشک سیاه (‏Rubus sanctus‏) در مراحل مختلف رسیدگی ‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران ‏

2 استاد، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران ‏

3 دانشیار، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران ‏

چکیده

مطالعه حاضر به‏منظور بررسی تنوع در ویژگی‌های آنتی‌اکسیدانی، ترکیبات فنلی، صفات کمی میوه و میزان رنگدانه‌های ژنوتیپ‌های وحشی تمشک‌سیاه ایران انجام‏گرفت. برای این منظور، چهار ژنوتیپ تمشک‌ سیاه شامل بابلسر، نمک‌آبرود، بندرگز و ناهارخوران متعلق به گونه Rubus sanctus که از شمال ایران جمع آوری شده بودند و در کلکسیون تمشک‌ سیاه دانشگاه شیراز نگهداری می‌شدند، انتخاب و در قالب آزمایش فاکتوریل بر پایه طرح بلوک‌های کامل تصادفی در چهار تکرار و در دو سال  متوالی مورد بررسی قرار‌گرفتند. سپس در سه مرحله مختلف رسیدگی میوه (سبز، قرمز، سیاه) از ژنوتیپ‌های مورد نظر نمونه تهیه‏شد. بر‏اساس نتایج تفاوت معنی‌داری بین ژنوتیپ‌های مطالعه‏شده از لحاظ صفات IC50 (50/25-65/46درصد)، فنل کل (53/350-67/224میلی‌گرم در 100 گرم ماده خشک)، فلاونوئید (37/812 - 71/531 میلی‌گرم در 100 گرم ماده خشک)، فلاونون (55/75-47/18 میلی‌گرم در 100 گرم ماده خشک)، آنتوسیانین (57/281- 23/92 میلی‌گرم در 100 گرم ماده تازه) به‌همراه دیگر صفات وجود‌ داشت. علاوه بر این، میزان ترکیبات فنلی و آنتی‌اکسیدان میوه سیاه رنگ در مقایسه با دیگر مراحل رسیدگی بیشترین مقدار بود. همچنین ارتباط مثبت و معنی‌داری بین میزان آنتی‌اکسیدان و ترکیبات فنلی مشاهده شد. ژنوتیپ بابلسر با دارا بودن بیشترین میزان فنل (53/350 میلی‌گرم در 100 گرم ماده خشک)،  آنتوسیانین (57/281 میلی‌گرم در 100 گرم ماده تازه)، فلاونون (55/75 میلی‌گرم در 100 گرم ماده خشک)، فلاونوئید (37/812 میلی‌گرم در 100 گرم ماده خشک) و کمترین میزان IC50 (25/46 درصد) به عنوان بهترین ژنوتیپ از لحاظ ارزش غذایی مشخص گردید.

کلیدواژه‌ها

عنوان مقاله [English]

Assessment of antioxidant activity, phenolic components, photosynthesis pigments ‎and fruit quantitative traits in four blackberry (Rubus sanctus) accessions at ‎different fruit maturity stages

نویسندگان [English]

  • Zahra Shams 1
  • Saied Eshghi 2
  • Enayatollah Tafazoli 2
  • Ali Gharaghani 3
1 Ph. D. Candidate, School of Agriculture, Shiraz University, Shiraz, Iran
2 Professor, School of Agriculture, Shiraz University, Shiraz, Iran
3 Associate Professor, School of Agriculture, Shiraz University, Shiraz, Iran
چکیده [English]

This study was conducted in order to evaluate the variation of blackberry accession of Iran in terms of antioxidant activity, phenolic components, fruit quantitative traits and photosynthesis pigments. For this regards, four accessions of wild blackberry including Babolsar, Naharkhoran, BandarGaz and NamakAbroud belongs to Rubus sanctus species, which collected from the northern area of Iran and has been kept in Blackberry Collection of Horticulture Department of Shiraz University were selected and subjected to factorial design based on randomized complete block designs with four replications in two consecutive years. Then, the fruit sanmples were collected in three fruit maturity stages (Black, Red and Green). The results indicated significant differences between accessions in terms of half maximal inhibitory concentration (IC50) (range, 46.25-65.50 %), total phenolic content (TPC) (range, 224.67-350.53 mg/100 g DW), flavonoid (range, 531.71-812.37 mg/100 g DW), flavanone (range, 18.47-75.55 mg/100 g DW), anthocyanin (range, 92.23-281.57 mg/100 g FW) along with other traits. Furthermore, the highest quantity of abtioxidant and phenolic compounds were observed in complete fruit maturity stages (Black) compared to other maturity stages. There was a positive and significant association between antioxidant activity and phenolic compounds. Babolsar genotype with highest quantity of phenol (350.53 mg/100 g DW), anthocyanin (281.57 mg/100 g FW), flavanone (75.55 mg/100 g DW), flavonoids (812.37 mg/100 g DW) and lowest amount of IC50 (46.25 %) identified as the best genotype in terms of nutritional value.

کلیدواژه‌ها [English]

  • Blackberry
  • germplasm
  • regression analysis‎

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علوم باغبانی ایران
دوره 52، شماره 1
خرداد 1400
صفحه 153-169

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  • تاریخ دریافت: 02 مرداد 1398
  • تاریخ بازنگری: 30 مهر 1398
  • تاریخ پذیرش: 17 آبان 1398

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