اثر سطوح مختلف شوری روی شاخص‌های رشد، جذب عناصر‌، فعالیت آنزیم‌های آنتی‌اکسیدانی و برخی صفات فیزیولوژیک ریشه و برگ پایه هیبرید GN15

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه تبریز

2 استاد، دانشکده کشاورزی، دانشگاه تبریز

3 محقق، مرکز تحقیقات کشاورزی استان آذربایجان‌شرقی

چکیده

به‌منظور بررسی اثرات سطوح مختلف شوری روی شاخص‌های رشد، میزان پرولین و کلروفیل، غلظت و کارایی جذب نیتروژن، پتاسیم، فسفر و سدیم برگ و غلظت سدیم ریشه پایه‌های هیبرید GN15 و همچنین میزان فعالیت سه آنزیم آنتی‌اکسیدانت، آزمایشی بر مبنای طرح کاملاً تصادفی با 4 سطح شوری صفر، 35، 70 و 105 میلی‌گرم بر لیتر کلریدسدیم و با 5 تکرار در سال 1394 انجام شد. غلظت‌های مختلف کلریدسدیم به گلدان‌های محتوی این پایه افزوده و پس از 100 روز تنش شوری، صفات مذکور اندازه‌گیری شد. نتایج نشان داد که با افزایش سطوح شوری، شاخص‌های رشد شامل طول و قطر گیاه، تعداد و سطح برگ، محتوای رطوبت نسبی و کلروفیل برگ کاهش می‌یابد که این کاهش در غلظت 35 میلی‌مولار کمتر ولی در غلظت 105 میلی‌مولار بسیار شدید بود. غلظت و کارایی جذب نیتروژن، پتاسیم و فسفر با افزایش غلظت کلریدسدیم کاهش و غلظت سدیم برگ و ریشه و کارایی جذب آن در برگ افزایش پیدا کرد. میزان پرولین ریشه و برگ نیز همزمان با افزایش غلظت نمک افزایش یافت؛ به‌طوری‌که مقدارش در غلظت 70 میلی‌مولار به بیشینه خود (39 و 30 میلی‌گرم در هر گرم وزن تر) رسید. فعالیت آنزیم‌های آنتی‌اکسیدانی نیز با افزایش غلظت نمک تا 70 میلی‌مولار روند افزایشی داشت به‌طوری‌که فعالیت SOD، POD و CAT در این تیمار برای ریشه به‌ترتیب 2/150، 6/22، 13 و برای برگ 8/183، 8/16 و 24 واحد در هر میلی‌گرم پروتئین بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of different levels of salinity on growth indices, mineral absorption, antioxidant enzymes activity and some physiological traits of roots and leaf in GN15 hybrid rootstocks

نویسندگان [English]

  • Akbar Angooti 1
  • Jafar Hajilou 2
  • Edris Hajali 1
  • Hossein Fathi 3
1 Former M. Sc. Student, Faculty of Agriculture, University of Tabriz, East Azarbaijan, Iran
2 Professor, Faculty of Agriculture, University of Tabriz, East Azarbaijan, Iran
3 Research, East Azarbaijan Agricultural Research Center, Iran
چکیده [English]

In order to investigate the effects of different levels of salinity on growth indices, proline and chlorophyll content, nitrogen concentration, potassium, phosphorus and sodium contents of leaf and sodium concentration of root of GN15 hybrid rootstocks, as well as the activity of three antioxidant enzymes, an experiment was conducted based on a completely randomized design with 4 salinity levels of 0, 35, 70 and 105 mg / L sodium chloride and 5 replicates in 1394. Different concentrations of sodium chloride were added to the pots containing these plants and after 100 days of salinity stress, these traits were measured. Results showed as increasing salinity levels, growth indices including plant length and diameter, leaf number and area, relative humidity content and leaf chlorophyll content decreased. This reduction was lower at concentration of 35 mm but was very severe at 105 mM. Concentration and efficiency uptake of nitrogen, potassium and phosphorus decreased with increasing sodium chloride concentrations, and increased the concentration of sodium in leaves and roots and its uptake efficiency in leaves. The amount of proline in the root and leaf increased simultaneously with increasing salt concentrations, so that its amount reached a maximum at concentration of 70 mM (39 and 30 mg / g fresh weight). The activity of antioxidant enzymes was also increased by increasing the salt concentration up to 70 mM, so that SOD, POD and CAT of roots in this treatment were 150.2, 22.6,13 and for leavs were 183.8, 16.8 and 24 U/mg protein, respectively.

کلیدواژه‌ها [English]

  • Catalase
  • Chlorophyll
  • Peroxidase
  • proline
  • superoxide dismutase

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  • تاریخ دریافت: 22 اسفند 1396
  • تاریخ بازنگری: 26 خرداد 1397
  • تاریخ پذیرش: 26 تیر 1397

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