بررسی تأثیر تنظیم‌کننده‌های رشد و امواج فراصوت بر ریشه‌زایی و پینه‌زایی سوسن چلچراغ (Lilium ledebourii Bioss.) در شرایط درون‌شیشه‌ای

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه محقق اردبیلی

2 استادیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی

3 دانشیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی

چکیده

این تحقیق به‌منظور بررسی تأثیر تیمار NAA و BA و امواج فراصوت بر ریشه‌زایی و پینه (کالوس‌)زایی ریزنمونه‌های سوسن چلچراغ (Lilium ledebourii Bioss.) در شرایط درون‌شیشه‌ای انجام شد. برای این منظور ریزنمونه‌های فلس پس از قرارگیری در حمام فراصوت با بسامد (فرکانس) 35 کیلوهرتز با زمان‌های 0، 5، 10، 20 و 30 ثانیه، در محیط کشت MS حاوی غلظت‌های 0، 01/0، 1/0 و 1 میلی‌گرم در لیتر NAA و BA به‌تنهایی و یا به‌صورت ترکیب با یکدیگر کشت شد. این تحقیق در چهار آزمایش جداگانه به ترتیب شامل ترکیب NAA و BA، NAA و فراصوت، BA و فراصوت و بهترین تیمارهای آزمایش اول همراه با فراصوت انجام گرفت. در آزمایش اول و دوم تیمار mg l-1 1 NAA بیشترین شمار ریشه و طول ریشه را داشت و در آزمایش سوم تیمار شاهد نتیجۀ بهتری در این زمینه داشت. در آزمایش دوم فراصوت ریشه‌زایی را تحریک نکرد، بااین‌حال در آزمایش سوم، در غلظت پایین BA فراصوت تأثیر مثبتی در ریشه‌زایی داشت و شمار ریشه‌های به‌دست‌آمده را تا 12-10 عدد افزایش داد، ولی در غلظت 0 و mg l-1  BA 1، تأثیر مثبتی در ریشه‌زایی نداشت. نتایج مربوط به پینه‌زایی نشان داد، تنظیم‌کننده‌های رشد و امواج فراصوت تأثیر معنی‌داری بر میزان پینه‌زایی ریزنمونه نداشتند. در این پژوهش غلظت mg l-11 NAA به‌تنهایی بهترین تیمار برای افزایش فراسنجه (پارامتر)های مربوط به ریشه‌زایی ریزنمونۀ فلس شناخته شد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of plant growth regulators and ultrasound treatments on in vitro rooting and callogenesis of Lilium ledebourii Boiss.

نویسندگان [English]

  • Zahra Azimzadeh 1
  • Mehdi Mohebodini 2
  • Esmaeil Chamani 3
1 Former M. Sc. Student, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran
2 Assistant Professor, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran
3 Assocaite Professor, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran
چکیده [English]

This study was performed to evaluate the effect of NAA, BA and ultrasound waves on in vitro rooting and callus induction of Lilium ledebourii. For this purpose, scale explants after exposure to an ultrasonic bath with a frequency of 35 kHz in times 0, 5, 10, 20 and 30 seconds were cultured on MS medium containing different concentrations (0, 0.01, 0.1 and 1 mg l-1) of NAA and BA alone or in combination with each other. This study was carried out in four experiments including combination of NAA and BA, NAA and ultrasound, BA and ultrasound, and the best treatments which achieved from first experiment with ultrasound, respectively. In the first and second experiment, 1 mg l-1 NAA treatments had the highest root induction and in the third experiment control treatment had the better result. In the second experiment, ultrasound did not stimulate root induction, however, in the third experiment, in 0 and 1 mg l-1 BA concentrations, ultrasound had no positive effect on root induction, but at low concentrations, BA increased the number of roots. Moreover, it was indicated that plant growth regulators and ultrasound had no significant effect on callus induction. In this study, concentration of 1 mg l-1 NAA was the best treatment for increasing of rooting parameters in scale explants.

کلیدواژه‌ها [English]

  • BA
  • NAA
  • explant
  • Scale

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سابقه مقاله

  • تاریخ دریافت: 21 تیر 1395
  • تاریخ بازنگری: 07 مهر 1395
  • تاریخ پذیرش: 07 مهر 1395

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