تأثیر اسید آسکوربیک بر برخی ویژگی‌های مورفولوژی و فیزیولوژی شمعدانی معطر (Pelargonium graveolens) در شرایط کمبود آهن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه لرستان

2 دانشیار، دانشکدۀ کشاورزی، دانشگاه لرستان

3 مربی، دانشگاه صنعتی خاتم‌الانبیاء بهبهان

چکیده

به‌منظور بررسی تأثیر اسید آسکوربیک در کاهش تأثیر وجود کمبود آهن، آزمایشی به‌صورت فاکتوریل بر پایۀ طرح کاملاً تصادفی با شش تکرار در گیاه شمعدانی معطر انجام شد. عامل‌ها شامل میزان آهن با سه سطح (0، 20 و 40 میکرو مولار) و اسیدآسکوربیک با سه سطح (0، 1 و 2 میلی­مولار) بود. قلمه­های ریشه­دار شده به‌صورت آبکشتی (هیدروپونیک) درون ماسه کاشت شده و پس از استقرار گیاهان تیمارهای آهن و اسید آسکوربیک به همراه محلول غذایی نیم­هوگلند اعمال شد. نتایج نشان داد، با کاهش میزان آهن ویژگی‌های رشد مانند ارتفاع گیاه، قطر ساقه، شمار و طول شاخه­های جانبی و سطح برگ در بوته کاهش یافت. با کاهش میزان آهن وزن‌ تر و خشک برگ، ساقه و پیکر رویشی کاهش یافته ولی میزان اسانس افزایش یافت. واکنش گیاه به اسیدآسکوربیک در تغذیه با آهن 0 و 20 میکرومولار با هم متفاوت بود. اسیدآسکوربیک (به‌ویژه با غلظت 1 میلی­مولار) باعث افزایش زیست‌توده (بیوماس) و میزان اسانس شد. با کاهش میزان آهن، عملکرد اسانس کاهش و با کاربرد اسید آسکوربیک افزایش یافت. کاهش آهن باعث کاهش سبزینه (کلروفیل)­ها و کاروتنوئیدها و کاربرد اسیدآسکوربیک باعث بهبود این ویژگی­­­ها شد. بنابر نتایج، کاربرد اسیدآسکوربیک توانست رشد و عملکرد گیاه شمعدانی معطر را در تیمار 20 میکرومولارآهن  بهبود بخشد اما تأثیر آن در شرایط آهن 0 میکرومولار بسیار کمتر بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of ascorbic acid on some morphological and physiological characteristics of Pelargonium graveolens under iron deficiency

نویسندگان [English]

  • Kobra Sepahvand 1
  • Abdolhossein Rezaei Nejad 2
  • Seyedeh Zahra Hosseini 3
1 Former M.Sc. Student, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
2 Associate Professor, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
3 Lecturer, Faculty of Basic Sciences, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran
چکیده [English]

In order to study the effect of ascorbic acid (AsA) on quantitative and qualitative characteristics of Pelargonium graveolens under iron (Fe) deficiency stress, a pot experiment was carried out. Three levels of Fe (0, 20 and 40 µM) and three levels of AsA (0, 1 and 2 mM) were combined factorially based on a completely randomized design with six replications. Uniform rooted cutting were planted in sand and grown hydroponically. After plant establishment, Fe and AsA treatments were applied through half-Hoagland nutrient solution. Results showed that as Fe in nutrient solution decreased, growth characters i.e. plant height, stem diameter, number and length of axillary shoots and leaf area decreased. Moreover, as Fe in nutrient solution decreased, leaf fresh and dry weight, stem fresh and dry weight, decreased, while, oil content increased. Growth responses to AsA was different in plants grown in nutrient solution with 0 µM Fe compared with those in plants grown in nutrient solution containing 20 µM Fe. Daily application of AsA (especially with 1 mM concentration in nutrient solution) increased biomass and oil content. Oil yield, Chlorophylls and carotenoids contents decreased with decreasing Fe, while, AsA increased them under Fe deficiency. Overall, AsA could improve growth and yield of geranium under 20 μM Fe nutrition, however, it was less effective at 0 μM Fe.

کلیدواژه‌ها [English]

  • Essential oil
  • plant nutrition
  • Soilless culture
  1. Allen, R. D. (1995). Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology 107, 1049-1054.
  2. Almaliotis, D., Velemis, D., Bladenopoulou, S. & Karapetsas, N. (2000). Leaf nutrient levels of strawberries (cv. Tudla) in relation to crop yield. In: IV International Strawberry Symposium 567 (pp. 447-450).
  3. Chen, Y., Clapp, C. E. & Magen, H. (2004). Mechanisms of plant growth stimulation by humic substances: The role of organo-iron complexes. Soil Science and Plant Nutrition, 50(7), 1089-1095.
  4. Eiasu, B. K., Steyn, J. M. & Soundy, P. (2009). Rose-scented geranium (Pelargonium capitatum× P. radens) growth and essential oil yield response to different soil water depletion regimes. Agricultural Water Management, 96(6), 991-1000.
  5. 5.       Khalid, K. A. (2006). Influence of water stress on growth, essential oil, and chemical composition of herbs (Ocimum sp.). International Agrophysics, 20(4), 289-296.
  6. Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Method in Enzymology. (eds. S. P. Colowick and N.O. Kaplan) Academic Press. New York, 48, 350-382.
  7. Lindsay, W. L. (1979). Chemical equilibria in soils. John Wiley and Sons Ltd. NewYork. USA.
  8. Lis-Balchin, M. (2003). 14 Essential oils from different Pelargonium species and cultivars: their chemical composition (using GC, GC/MS) and appearance of trichomes (under EM). Geranium and Pelargonium: History of Nomenclature, Usage and Cultivation, 147.
  9. Malakouti, M. J. & Tehrani, M. M. (1999). Effect of micronutrients application on yield and quality of agricultural products. Tarbiat Modares University Press. 300pp. (in Farsi)
  10. Malatova, K., Hitimana, N., Niyibizi, T., Simon, J. E. & Juliani, H. R. (2011). Optimization of harvest regime and post-harvest handling in geranium production to maximize essential oil yield in Rwanda. Industrial Crops and Products, 34(2), 1348-1352.
  11. Marschner, H. (2011). Marschner's mineral nutrition of higher plants. Academic press. New York. USA.
  12. Mengel, K. & Kirkby, E. A. (1987). Principles of plant nutrition (No. Ed. 4). International Potash Institute. Bern, Switzerland.
  13. Mori, S. (1999). Iron acquisition by plants. Current opinion in plant biology, 2(3), 250-253.
  14. Motsa, N. M., Soundy, P., Steyn, J. M., Learmonth, R. A., Mojela, N. & Teubes, C. (2006). Plant shoot age and temperature effects on essential oil yield and oil composition of rose-scented geranium (Pelargonium sp.) grown in South Africa. Journal of Essential Oil Research, 18, 106-110.
  15. Rajeswara Rao, B., Kaul, P. N., Mallavarapu, G. R. & Ramesh, S. (1996). Effect of seasonal climatic changes on biomass yield and terpenoid composition of rose-scented geranium (Pelargonium species). Biochemical Systematics and Ecology, 24(7), 627-635.
  16. Ramírez, L., Bartoli, C. G. & Lamattina, L. (2013). Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency. Journal of Experimental Botany, 64(11), 3169-3178.
  17. Rezaei Nejad, A. & Ismaili, A. (2014). Changes in growth, essential oil yield and composition of geranium (Pelargonium graveolens L.) as affected by growing media. Journal of the science of food and agriculture, 94(5), 905-910.
  18. Rouphael, Y., Cardarelli, M., Rea, E. & Colla, G. (2008). The influence of irrigation system and nutrient solution concentration on potted geranium production under various conditions of radiation and temperature. Scientia horticulturae, 118(4), 328-337.
  19. Said-Al Ahl, H. A. H. & Mahmoud, A. A. (2010). Effect of zinc and/or iron foliar application on growth and essential oil of sweet basil (Ocimum basilicum L.) under salt stress. Ozean Journal of Applied Science, 3(1), 97-110.
  20. Shalata, A. & Neumann, P. M. (2001). Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. Journal of Experimental Botany, 52(364), 2207-2211.
  21. Santos, C. S., Roriz, M., Carvalho, S. M. & Vasconcelos, M. W. (2015). Iron partitioning at an early growth stage impacts iron deficiency responses in soybean plants (Glycine max L.). Frontiers in Plant Science, 6.
  22. Simon, J. E., Reiss-Bubenheim, D., Joly, R. J. & Charles, D. J. (1992). Water stress-induced alterations in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4(1), 71-75.
  23. Singh, R. K., Singh, R. P. & Singh, R. S. (2003). Effect of iron on herbage and oil yield of lemon grass (Cymbopogon flexuosus). Crop Research-Hisar, 26(1), 185-187.
  24. Singh, D. V., Srivastava, G. C. & Abdin, M. Z. (2001). Amelioration of negative effect of water stress in Cassia angustifolia by benzyladenine and/or ascorbic acid. Biologia Plantarum, 44(1), 141-143.
  25. Tewari, R. K., Kumar, P. & Sharma, P. N. (2005). Signs of oxidative stress in the chlorotic leaves of iron starved plants. Plant Science 169, 1037-1045.
  26. Veljovic-Jovanovic, S. D., Pignocchi, C., Noctor, G. & Foyer, C. H. (2001). Low ascorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system. Plant Physiology, 127(2), 426-435.
  27. Zha, Q., Wang, Y., Zhang, X. Z. & Han, Z. H. (2014). Both immanently high active iron contents and increased root ferrous uptake in response to low iron stress contribute to the iron deficiency tolerance in Malus xiaojinensis. Plant Science, 214, 47-56.